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Abstract：
Background: Chronic rhinosinusitis (CRS) is an inflammatory condition classified into chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP). Th cells manage inflammatory cells in CRS. Suppressor of Cytokine Signaling (SOCS) proteins regulate Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway in Th cells by polarizing toward Th1, Th2, and Th17 cells. This study evaluated the levels of SOCS1,3,5 in CRS patients to find associations with Th cells. Methods: In this cross-sectional study, 20 CRSwNP patients, 12 CRSsNP patients, and 12 controls participated. The infiltration of CD4+ T cells was determined using immunohistochemistry. The expression of specific transcription factors and SOCS proteins was assessed using real-time PCR. Cytokine levels were evaluated using ELISA. SOCS protein levels were investigated using western blot analysis. Results: The expression of SOCS3 increased in the CRSwNP group compared to CRSsNP and control groups (p <0.001). SOCS3 protein levels increased in the CRSwNP group compared to CRSsNP (p <0.05) and control (p <0.001) groups. Although there was a significant difference in SOCS5 expression between CRSsNP and control groups, SOCS5 protein levels were significantly different between CRSsNP and control (p <0.001) and CRSwNP (p <0.05) groups. Conclusions: Targeted therapies may be suggested for CRS by modulating SOCS3 and SOCS5 proteins that are responsible for polarization of Th cells toward Th2 or Th1 cells, respectively. JAK-STAT pathway targeting, which encompasses numerous cells, can be limited to SOCS proteins to more effectively orchestrate Th cell differentiation.
摘要：
背景：慢性鼻-鼻窦炎（CRS）是一种炎症性疾病，分为伴鼻息肉的慢性鼻-鼻窦炎（CRSwNP）和不伴鼻息肉的慢性鼻-鼻窦炎（CRSsNP）。Th细胞管理CRS中的炎性细胞。细胞因子信号抑制蛋白（SOCS）通过向Th1，Th2和Th17细胞极化来调节Th细胞中的Janus激酶（JAK）-信号转导子和转录激活因子（STAT）途径。这项研究评估了CRS患者中SOCS1,3,5的水平，以发现与Th细胞的关联。方法：在这项横断面研究中，20名CRSwNP患者，12名CRSsNP患者，和12个控制参与。使用免疫组织化学确定CD4+T细胞的浸润。使用实时PCR评估特定转录因子和SOCS蛋白的表达。使用ELISA评估细胞因子水平。使用蛋白质印迹分析研究SOCS蛋白水平。结果：与CRSsNP组和对照组相比，CRSwNP组中SOCS3的表达增加（p<0.001）。与CRSsNP组(p<0.05)和对照组(p<0.001)相比,CRSwNP组的SOCS3蛋白水平增加。尽管CRSsNP组和对照组之间的SOCS5表达存在显着差异，SOCS5蛋白水平在CRSsNP与对照组(p<0.001)和CRSwNP(p<0.05)组之间显著不同。结论：可以通过调节SOCS3和SOCS5蛋白来建议CRS的靶向治疗，这些蛋白负责Th细胞向Th2或Th1细胞的极化。分别。JAK-STAT通路靶向,包括许多细胞，可以限于SOCS蛋白以更有效地协调Th细胞分化。
