PDF(Pubmed)
Abstract：
Wolbachia is an obligate intracellular α-proteobacterium which commonly infects arthropods and filarial nematodes. Different strains of Wolbachia are capable of a wide range of regulatory manipulations in many hosts and modulate host cellular differentiation to influence host reproduction. The genetic basis for the majority of these phenotypes is unknown. The wWil strain from the neotropical fruit fly, Drosophila willistoni, exhibits a remarkably high affinity for host germline-derived cells relative to the soma. This trait could be leveraged for understanding how Wolbachia influences the host germline and for controlling host populations in the field. To further the use of this strain in biological and biomedical research, we sequenced the genome of the wWil strain isolated from host cell culture cells. Here, we present the first high quality nanopore assembly of wWil, the Wolbachia endosymbiont of D. willistoni. Our assembly resulted in a circular genome of 1.27 Mb with a BUSCO completeness score of 99.7%. Consistent with other insect-associated Wolbachia strains, comparative genomic analysis revealed that wWil has a highly mosaic genome relative to the closely related wMel strain from Drosophila melanogaster.
摘要：
Wolbachia是一种专性的细胞内?-蛋白细菌，通常感染节肢动物和丝虫线虫。Wolbachia的不同菌株能够在许多宿主中进行广泛的调节操作，并调节宿主细胞分化以影响宿主繁殖。大多数这些表型的遗传基础是未知的。来自新热带果蝇的wWil菌株，果蝇willistoni,相对于体细胞，对宿主生殖系衍生细胞表现出非常高的亲和力。可以利用此性状来了解Wolbachia如何影响宿主种系以及控制田间宿主种群。为了进一步将该菌株用于生物和生物医学研究，我们对从宿主细胞培养细胞中分离的wWil菌株的基因组进行了测序。这里,我们提出了第一个高质量的WWIL纳米孔组装体，D.willistoni的Wolbachia内共生体.我们的组装产生了1.27Mb的环状基因组，BUSCO完整性评分为99.7％。与其他与昆虫相关的Wolbachia菌株一致，比较基因组分析显示，相对于果蝇中密切相关的wMel菌株，wWil具有高度镶嵌的基因组。
