PDF(Pubmed)
Abstract：
UNASSIGNED: This study aimed to explore the anti-oxidative and anti-inflammatory properties of Lactococcus lactis subsp. lactis HFY14 (LLSLHFY14) and investigate its effects on the intestinal barrier, cranial nerve, and motor function in mice treated with antibiotics.
UNASSIGNED: Mice were administered an antibiotic mixture (neomycin 5 mg/mL, vancomycin 25 mg/mL, amphotericin B 0.1 mg/mL, ampicillin 10 mg/mL, metronidazole file 5 mg/mL, and lipopolysaccharide 1.5 μg/mL) intraperitoneally, and oxidative stress and inflammatory markers in the serum and brain tissues, and liver index were measured. H&E staining was performed to detect pathological alterations in brain tissues. The expression of intestinal-barrier-related genes and that of genes involved in inflammatory pathways in the brain were detected using polymerase chain reaction (PCR).
UNASSIGNED: LLSLHFY14 administration extended the weight-loaded swimming and running times of mice and decreased the liver index. Moreover, the levels of malondialdehyde (MDA), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) in the serum and brain tissue were reduced, whereas those of superoxide dismutase (SOD), glutathione (GSH), and interleukin-10 (IL-10) were elevated. Elevated brain expression of the protein kinase B (AKT)/cAMP-response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF)/extracellular signal-regulated kinase 1 (ERK1) pathway, decreased brain expression of the IL-6 gene, and elevated cecum expression of zonula occludens-1 (ZO-1), occludin-1, and claudin-1 genes were noted. LLSLHFY14 supplementation significantly increased Bacteroidetes expression but decreased Firmicutes expression, thus increasing the Bacteroidetes/Firmicutes ratio.
UNASSIGNED: Overall, LLSLHFY14 supplementation ameliorated antibiotic-induced oxidative stress and inflammation in the mouse central nervous system, intestinal barrier dysfunction, and increased motor function, thus confirming its potential application as probiotics.
UNASSIGNED: Mice were administered an antibiotic mixture (neomycin 5 mg/mL, vancomycin 25 mg/mL, amphotericin B 0.1 mg/mL, ampicillin 10 mg/mL, metronidazole file 5 mg/mL, and lipopolysaccharide 1.5 μg/mL) intraperitoneally, and oxidative stress and inflammatory markers in the serum and brain tissues, and liver index were measured. H&E staining was performed to detect pathological alterations in brain tissues. The expression of intestinal-barrier-related genes and that of genes involved in inflammatory pathways in the brain were detected using polymerase chain reaction (PCR).
UNASSIGNED: LLSLHFY14 administration extended the weight-loaded swimming and running times of mice and decreased the liver index. Moreover, the levels of malondialdehyde (MDA), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) in the serum and brain tissue were reduced, whereas those of superoxide dismutase (SOD), glutathione (GSH), and interleukin-10 (IL-10) were elevated. Elevated brain expression of the protein kinase B (AKT)/cAMP-response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF)/extracellular signal-regulated kinase 1 (ERK1) pathway, decreased brain expression of the IL-6 gene, and elevated cecum expression of zonula occludens-1 (ZO-1), occludin-1, and claudin-1 genes were noted. LLSLHFY14 supplementation significantly increased Bacteroidetes expression but decreased Firmicutes expression, thus increasing the Bacteroidetes/Firmicutes ratio.
UNASSIGNED: Overall, LLSLHFY14 supplementation ameliorated antibiotic-induced oxidative stress and inflammation in the mouse central nervous system, intestinal barrier dysfunction, and increased motor function, thus confirming its potential application as probiotics.
摘要：
■本研究旨在探讨乳酸乳球菌亚种的抗氧化和抗炎特性。乳酸HFY14（LLSLHFY14），并研究其对肠道屏障的影响，颅神经,和用抗生素治疗的小鼠的运动功能。
■给小鼠服用抗生素混合物（新霉素5mg/mL，万古霉素25mg/mL，两性霉素B0.1mg/mL，氨苄青霉素10mg/mL，甲硝唑文件5毫克/毫升，和脂多糖1.5μg/mL）腹膜内，血清和脑组织中的氧化应激和炎症标志物，测量肝脏指数。进行H&E染色以检测脑组织中的病理改变。使用聚合酶链反应（PCR）检测大脑中肠屏障相关基因和炎症途径相关基因的表达。
■LLSLHFY14给药延长了小鼠的负重游泳和跑步时间，并降低了肝脏指数。此外，丙二醛（MDA）的水平，白细胞介素-6（IL-6），血清和脑组织中肿瘤坏死因子α（TNF-α）均降低，而超氧化物歧化酶(SOD)，谷胱甘肽(GSH),白细胞介素-10（IL-10）升高。蛋白激酶B（AKT）/cAMP反应元件结合蛋白（CREB）/脑源性神经营养因子（BDNF）/细胞外信号调节激酶1（ERK1）途径的脑表达升高，IL-6基因的大脑表达减少，盲肠小带1（ZO-1）的表达升高，注意到occludin-1和claudin-1基因。LLSLHFY14补充显着增加了拟杆菌的表达，但降低了Firmicutes的表达，从而增加拟杆菌/厚壁菌的比例。
■总的来说，LLSLHFY14补充剂改善了抗生素诱导的小鼠中枢神经系统的氧化应激和炎症，肠屏障功能障碍，增加了运动功能，从而证实了其作为益生菌的潜在应用。
■给小鼠服用抗生素混合物（新霉素5mg/mL，万古霉素25mg/mL，两性霉素B0.1mg/mL，氨苄青霉素10mg/mL，甲硝唑文件5毫克/毫升，和脂多糖1.5μg/mL）腹膜内，血清和脑组织中的氧化应激和炎症标志物，测量肝脏指数。进行H&E染色以检测脑组织中的病理改变。使用聚合酶链反应（PCR）检测大脑中肠屏障相关基因和炎症途径相关基因的表达。
■LLSLHFY14给药延长了小鼠的负重游泳和跑步时间，并降低了肝脏指数。此外，丙二醛（MDA）的水平，白细胞介素-6（IL-6），血清和脑组织中肿瘤坏死因子α（TNF-α）均降低，而超氧化物歧化酶(SOD)，谷胱甘肽(GSH),白细胞介素-10（IL-10）升高。蛋白激酶B（AKT）/cAMP反应元件结合蛋白（CREB）/脑源性神经营养因子（BDNF）/细胞外信号调节激酶1（ERK1）途径的脑表达升高，IL-6基因的大脑表达减少，盲肠小带1（ZO-1）的表达升高，注意到occludin-1和claudin-1基因。LLSLHFY14补充显着增加了拟杆菌的表达，但降低了Firmicutes的表达，从而增加拟杆菌/厚壁菌的比例。
■总的来说，LLSLHFY14补充剂改善了抗生素诱导的小鼠中枢神经系统的氧化应激和炎症，肠屏障功能障碍，增加了运动功能，从而证实了其作为益生菌的潜在应用。
