PDF(Pubmed)
Abstract：
UNASSIGNED: Acinetobacter baumannii (A. baumannii) is an important opportunistic pathogen causing nosocomial infection in the clinic. The occurrence rate of antibiotic resistance is increasing year by year, resulting in a highly serious situation of bacterial resistance.
UNASSIGNED: To better understand the local epidemiology of multidrug-resistant A. baumannii, an investigation was conducted on the antibiotic resistance of different types of A. baumannii and its relationship with the genes of A. baumannii. Furthermore, the molecular mechanism underlying antibiotic resistance in A. baumannii was investigated through transcriptome analysis.
UNASSIGNED: These results showed that a total of 9 STs were detected. It was found that 99% of the strains isolated in the hospital belonged to the same STs, and the clone complex CC208 was widely distributed in various departments and all kinds of samples. Furthermore, these A. baumannii strains showed high resistance to ertapenem, biapenem, meropenem, and imipenem, among which the resistance to ertapenem was the strongest. The detection rate of bla OXA-51 gene in these carbapenem resistance A. baumannii (CRAB) reached 100%; Additionally, the transcriptome results showed that the resistance genes were up-regulated in resistance strains, and these genes involved in biofilm formation, efflux pumps, peptidoglycan biosynthesis, and chaperonin synthesis.
UNASSIGNED: These results suggest that the CC208 STs were the main clonal complex, and showed high carbapenem antibiotic resistance. All these resistant strains were distributed in various departments, but most of them were distributed in intensive care units (ICU). The bla OXA-23 was the main antibiotic resistance genotype; In summary, the epidemic trend of clinical A. baumannii in Guiyang, China was analyzed from the molecular level, and the resistance mechanism of A. baumannii to carbapenem antibiotics was analyzed with transcriptome, which provided a theoretical basis for better control of A. baumannii.
UNASSIGNED: To better understand the local epidemiology of multidrug-resistant A. baumannii, an investigation was conducted on the antibiotic resistance of different types of A. baumannii and its relationship with the genes of A. baumannii. Furthermore, the molecular mechanism underlying antibiotic resistance in A. baumannii was investigated through transcriptome analysis.
UNASSIGNED: These results showed that a total of 9 STs were detected. It was found that 99% of the strains isolated in the hospital belonged to the same STs, and the clone complex CC208 was widely distributed in various departments and all kinds of samples. Furthermore, these A. baumannii strains showed high resistance to ertapenem, biapenem, meropenem, and imipenem, among which the resistance to ertapenem was the strongest. The detection rate of bla OXA-51 gene in these carbapenem resistance A. baumannii (CRAB) reached 100%; Additionally, the transcriptome results showed that the resistance genes were up-regulated in resistance strains, and these genes involved in biofilm formation, efflux pumps, peptidoglycan biosynthesis, and chaperonin synthesis.
UNASSIGNED: These results suggest that the CC208 STs were the main clonal complex, and showed high carbapenem antibiotic resistance. All these resistant strains were distributed in various departments, but most of them were distributed in intensive care units (ICU). The bla OXA-23 was the main antibiotic resistance genotype; In summary, the epidemic trend of clinical A. baumannii in Guiyang, China was analyzed from the molecular level, and the resistance mechanism of A. baumannii to carbapenem antibiotics was analyzed with transcriptome, which provided a theoretical basis for better control of A. baumannii.
摘要：
■鲍曼不动杆菌(A.鲍曼不动杆菌）是临床上引起医院感染的重要机会性病原体。抗生素耐药性的发生率逐年上升,导致严重的细菌耐药性。
■为了更好地了解多药耐药鲍曼不动杆菌的当地流行病学，研究了不同类型鲍曼不动杆菌的耐药性及其与鲍曼不动杆菌基因的关系。此外，通过转录组分析研究鲍曼不动杆菌耐药性的分子机制。
■这些结果表明，共检测到9个ST。结果发现，在医院中分离出的99％的菌株属于相同的STs，克隆复合体CC208广泛分布于各个部门和各类样品中。此外，这些鲍曼不动杆菌菌株对厄他培南表现出很高的抗性，比阿培南,美罗培南,还有亚胺培南,其中对厄他培南的抵抗力最强。blaOXA-51基因在这些耐碳青霉烯类鲍曼不动杆菌(CRAB)中的检出率达到100%;转录组结果表明，抗性菌株中的抗性基因上调，这些参与生物膜形成的基因，外排泵,肽聚糖生物合成，和伴奏蛋白合成。
■这些结果表明，CC208STs是主要的克隆复合物，并表现出高的碳青霉烯类抗生素耐药性。所有这些耐药菌株分布在各个部门，但其中大部分分布在重症监护病房(ICU).BlaOXA-23是主要的抗生素抗性基因型；总之，贵阳市鲍曼不动杆菌临床流行趋势,从分子水平分析中国，用转录组分析鲍曼不动杆菌对碳青霉烯类抗生素的耐药机制,为更好地控制鲍曼不动杆菌提供了理论依据。
■为了更好地了解多药耐药鲍曼不动杆菌的当地流行病学，研究了不同类型鲍曼不动杆菌的耐药性及其与鲍曼不动杆菌基因的关系。此外，通过转录组分析研究鲍曼不动杆菌耐药性的分子机制。
■这些结果表明，共检测到9个ST。结果发现，在医院中分离出的99％的菌株属于相同的STs，克隆复合体CC208广泛分布于各个部门和各类样品中。此外，这些鲍曼不动杆菌菌株对厄他培南表现出很高的抗性，比阿培南,美罗培南,还有亚胺培南,其中对厄他培南的抵抗力最强。blaOXA-51基因在这些耐碳青霉烯类鲍曼不动杆菌(CRAB)中的检出率达到100%;转录组结果表明，抗性菌株中的抗性基因上调，这些参与生物膜形成的基因，外排泵,肽聚糖生物合成，和伴奏蛋白合成。
■这些结果表明，CC208STs是主要的克隆复合物，并表现出高的碳青霉烯类抗生素耐药性。所有这些耐药菌株分布在各个部门，但其中大部分分布在重症监护病房(ICU).BlaOXA-23是主要的抗生素抗性基因型；总之，贵阳市鲍曼不动杆菌临床流行趋势,从分子水平分析中国，用转录组分析鲍曼不动杆菌对碳青霉烯类抗生素的耐药机制,为更好地控制鲍曼不动杆菌提供了理论依据。
