PDF(Pubmed)
Abstract：
Bacterial endophytes dwelling in medicinal plants represent an as yet underexplored source of bioactive natural products with the potential to be developed into drugs against various human diseases. For the first time, several Streptomyces spp. were isolated from the rare and endangered traditional medicinal plant Leontopodium nivale ssp. alpinum, also known as Edelweiss. In the search for novel natural products, nine endophytic Streptomyces spp. from Edelweiss were investigated via genome sequencing and analysis, followed by fermentation in different media and investigation of secondary metabolomes. A total of 214 secondary metabolite biosynthetic gene clusters (BGCs), of which 35 are presumably unique, were identified by the bioinformatics tool antiSMASH in the genomes of these isolates. LC-MS analyses of the secondary metabolomes of these isolates revealed their potential to produce both known and presumably novel secondary metabolites, whereby most of the identified molecules could be linked to their cognate BGCs. This work sets the stage for further investigation of endophytic streptomycetes from Edelweiss aimed at the discovery and characterization of novel bioactive natural products.
摘要：
居住在药用植物中的内生细菌代表了生物活性天然产物的尚未开发的来源，具有开发成针对各种人类疾病的药物的潜力。第一次,几种链霉菌属。从稀有和濒危的传统药用植物益母草中分离出来。alpinum,也被称为雪绒花。在寻找新的天然产品时，九个内生链霉菌。通过基因组测序和分析对雪绒花进行了研究，然后在不同培养基中进行发酵和次生代谢组的研究。共有214个次级代谢产物生物合成基因簇(BGC)，其中35个大概是独一无二的，通过生物信息学工具antiSMASH在这些分离株的基因组中鉴定。这些分离物的次级代谢组的LC-MS分析揭示了它们产生已知和可能是新的次级代谢产物的潜力。由此大多数鉴定的分子可以与其同源的BGC连接。这项工作为进一步研究Edelweiss的内生链霉菌奠定了基础，旨在发现和表征新型生物活性天然产物。
