PDF(Pubmed)
Abstract：
UNASSIGNED: The dissemination of carbapenem-resistant Enterobacteriales (CRE) in nosocomial settings is primarily associated with the horizontal transfer of plasmids. However, limited research has focused on the in-host transferability of carbapenem resistance. In this study, ten isolates were collected from gut specimens of five individuals, each hosting two different species, including Escherichia coli, Klebsiella pneumoniae, Klebsiella aerogenes, Enterobacter cloacae, or Citrobacter koseri.
UNASSIGNED: Species identification and antimicrobial susceptibility were determined by MALDI-TOF MS and broth microdilution method. Carbapenemase genes were detected and localized using PCR, S1-PFGE and southern blot. The transferability of carbapenemase genes between species was investigated through filter mating experiments, and the genetic contexts of the plasmids were analyzed using whole genome sequencing.
UNASSIGNED: Our results revealed that each of the ten isolates harbored a carbapenemase gene, including bla NDM-5, bla NDM-1, or bla KPC-2, on a plasmid. Five different plasmids were successfully transferred to recipient cells of E. coli, K. pneumoniae or A. baumannii by transconjugation. The genetic contexts of the carbapenemase gene were remarkably similar between the two CRE isolates from each individual. This study highlights the potential for interspecies plasmid transmission in human gut, emphasizing the colonization of CRE as a significant risk factor for the dissemination of carbapenemase genes within the host. These findings underscore the need for appropriate intestinal CRE screening and colonization prevention.
UNASSIGNED: Species identification and antimicrobial susceptibility were determined by MALDI-TOF MS and broth microdilution method. Carbapenemase genes were detected and localized using PCR, S1-PFGE and southern blot. The transferability of carbapenemase genes between species was investigated through filter mating experiments, and the genetic contexts of the plasmids were analyzed using whole genome sequencing.
UNASSIGNED: Our results revealed that each of the ten isolates harbored a carbapenemase gene, including bla NDM-5, bla NDM-1, or bla KPC-2, on a plasmid. Five different plasmids were successfully transferred to recipient cells of E. coli, K. pneumoniae or A. baumannii by transconjugation. The genetic contexts of the carbapenemase gene were remarkably similar between the two CRE isolates from each individual. This study highlights the potential for interspecies plasmid transmission in human gut, emphasizing the colonization of CRE as a significant risk factor for the dissemination of carbapenemase genes within the host. These findings underscore the need for appropriate intestinal CRE screening and colonization prevention.
摘要：
■耐碳青霉烯的肠杆菌（CRE）在医院环境中的传播主要与质粒的水平转移有关。然而,有限的研究集中在碳青霉烯抗性的宿主内转移性上。在这项研究中,从五个个体的肠道标本中收集了十个分离株，每个都有两个不同的物种，包括大肠杆菌,肺炎克雷伯菌,产气克雷伯菌,阴沟肠杆菌,或者柠檬酸杆菌koseri.
■通过MALDI-TOFMS和肉汤微量稀释法测定物种鉴定和抗菌敏感性。使用PCR检测和定位碳青霉烯酶基因，S1-PFGE和Southern印迹。通过过滤器交配实验研究了碳青霉烯酶基因在物种之间的可转移性，使用全基因组测序分析了质粒的遗传背景。
■我们的结果表明，十种分离株中的每一种都含有碳青霉烯酶基因，包括质粒上的blaNDM-5、blaNDM-1或blaKPC-2。五种不同的质粒被成功地转移到大肠杆菌的受体细胞中，肺炎克雷伯氏菌或鲍曼不动杆菌通过转偶联。碳青霉烯酶基因的遗传背景在来自每个个体的两个CRE分离株之间非常相似。这项研究强调了人类肠道中种间质粒传播的潜力，强调CRE的定植是碳青霉烯酶基因在宿主内传播的重要危险因素。这些发现强调需要适当的肠道CRE筛查和定植预防。
■通过MALDI-TOFMS和肉汤微量稀释法测定物种鉴定和抗菌敏感性。使用PCR检测和定位碳青霉烯酶基因，S1-PFGE和Southern印迹。通过过滤器交配实验研究了碳青霉烯酶基因在物种之间的可转移性，使用全基因组测序分析了质粒的遗传背景。
■我们的结果表明，十种分离株中的每一种都含有碳青霉烯酶基因，包括质粒上的blaNDM-5、blaNDM-1或blaKPC-2。五种不同的质粒被成功地转移到大肠杆菌的受体细胞中，肺炎克雷伯氏菌或鲍曼不动杆菌通过转偶联。碳青霉烯酶基因的遗传背景在来自每个个体的两个CRE分离株之间非常相似。这项研究强调了人类肠道中种间质粒传播的潜力，强调CRE的定植是碳青霉烯酶基因在宿主内传播的重要危险因素。这些发现强调需要适当的肠道CRE筛查和定植预防。
