PDF(Pubmed)
Abstract：
UNASSIGNED: The Stenotrophomonas maltophilia complex (Smc) has emerged as a significant nosocomial pathogen contributing to increased mortality rates, particularly in case of bloodstream infections.
UNASSIGNED: This study employed whole-genome sequencing (WGS) to assess the genetic diversity, antimicrobial resistance profiles, molecular epidemiology and frequencies of virulence genes among 55 S. maltophilia isolates obtained from bacteremic cases over a 9-year period.
UNASSIGNED: Based on the threshold of 95% average nucleotide identity (ANI) and 70% digital DNA-DNA hybridization (dDDH) for genospecies delineation, we classified 37 isolates into 6 known species, all belonging to the Smc. The remaining 18 isolates sequenced in this study were assigned to 6 new genomospecies. Among the 55 isolates, we identified 44 different sequence types (STs), comprising 22 known and 22 novel allele combinations. The resistance rate of Smc against trimethoprim-sulfamethoxazole (TMP/SMX) was found to be 3.6%, with the sul1 and class one integron integrase genes (intI) detected in these isolates. All Smc isolates were susceptible to minocycline. Furthermore, all Smc strains harbored the motA, pilU, smf-1 and Stmpr2 genes. Genomospecies 1 (100%, n = 9), Stenotrophomonas maltophilia (84.21%, n = 19) and Stenotrophomonas sepilia (71.43%, n = 7) demonstrated a higher percentage of the afaD gene, which was also associated with a higher separation rate. In addition to motA, pilU, smf-1, and Stmpr2 genes, all S. maltophilia strains (100%) contained entA, gspD, KatA, and stmPr1 genes, while all genomospecies 1 strains (100%) contained afaD, entA, gspD, and KatA genes.
UNASSIGNED: Our study highlights the genetic diversity among Smc isolates from patients with bacteremia, revealing 22 novel ST types, 58 new alleles and 6 new genomospecies. S. maltophilia and S. pavanii were found to carry more virulence factors, emphasizing the importance of accurate strain identification. Minocycline emerged as a promising alternative antibiotic for patients who were resistant to TMP/SMX.
UNASSIGNED: This study employed whole-genome sequencing (WGS) to assess the genetic diversity, antimicrobial resistance profiles, molecular epidemiology and frequencies of virulence genes among 55 S. maltophilia isolates obtained from bacteremic cases over a 9-year period.
UNASSIGNED: Based on the threshold of 95% average nucleotide identity (ANI) and 70% digital DNA-DNA hybridization (dDDH) for genospecies delineation, we classified 37 isolates into 6 known species, all belonging to the Smc. The remaining 18 isolates sequenced in this study were assigned to 6 new genomospecies. Among the 55 isolates, we identified 44 different sequence types (STs), comprising 22 known and 22 novel allele combinations. The resistance rate of Smc against trimethoprim-sulfamethoxazole (TMP/SMX) was found to be 3.6%, with the sul1 and class one integron integrase genes (intI) detected in these isolates. All Smc isolates were susceptible to minocycline. Furthermore, all Smc strains harbored the motA, pilU, smf-1 and Stmpr2 genes. Genomospecies 1 (100%, n = 9), Stenotrophomonas maltophilia (84.21%, n = 19) and Stenotrophomonas sepilia (71.43%, n = 7) demonstrated a higher percentage of the afaD gene, which was also associated with a higher separation rate. In addition to motA, pilU, smf-1, and Stmpr2 genes, all S. maltophilia strains (100%) contained entA, gspD, KatA, and stmPr1 genes, while all genomospecies 1 strains (100%) contained afaD, entA, gspD, and KatA genes.
UNASSIGNED: Our study highlights the genetic diversity among Smc isolates from patients with bacteremia, revealing 22 novel ST types, 58 new alleles and 6 new genomospecies. S. maltophilia and S. pavanii were found to carry more virulence factors, emphasizing the importance of accurate strain identification. Minocycline emerged as a promising alternative antibiotic for patients who were resistant to TMP/SMX.
摘要：
■嗜麦芽窄食单胞菌复合体（Smc）已成为导致死亡率增加的重要医院病原体，特别是在血液感染的情况下。
■这项研究采用全基因组测序（WGS）来评估遗传多样性，抗菌素耐药性概况，从9年的菌血症病例中获得的55株嗜麦芽嗜血杆菌分离株的分子流行病学和毒力基因频率。
■基于95％平均核苷酸同一性（ANI）和70％数字DNA-DNA杂交（dDDH）的阈值，我们将37个分离株分为6个已知物种，都属于Smc。在这项研究中测序的其余18个分离株被分配给6个新的基因组物种。在55个分离株中，我们确定了44种不同的序列类型(ST)，包括22个已知的和22个新的等位基因组合。Smc对甲氧苄啶-磺胺甲恶唑(TMP/SMX)的耐药率为3.6%,在这些分离物中检测到sul1和一类整合子整合酶基因（intI）。所有Smc分离株都对米诺环素敏感。此外，所有Smc菌株都带有mota，pilu,smf-1和Stmpr2基因。同源物种1（100%，n=9),嗜麦芽窄食单胞菌(84.21%,n=19)和窄食单胞菌(71.43%，n=7)表明afaD基因的百分比较高，这也与较高的分离率有关。除了mota,pilu,smf-1和Stmpr2基因，所有嗜麦芽窄食链球菌菌株(100%)都含有entA，gspD,卡塔,和stmPr1基因，虽然所有基因物种1菌株(100%)都含有afaD，entA,gspD,和KatA基因.
■我们的研究强调了来自菌血症患者的Smc分离株的遗传多样性，揭示了22种新颖的ST类型，58个新等位基因和6个新基因组。研究发现嗜麦芽窄食链球菌和巴氏链球菌携带更多的毒力因子，强调准确菌株识别的重要性。对于对TMP/SMX耐药的患者，米诺环素是一种有前途的替代抗生素。
■这项研究采用全基因组测序（WGS）来评估遗传多样性，抗菌素耐药性概况，从9年的菌血症病例中获得的55株嗜麦芽嗜血杆菌分离株的分子流行病学和毒力基因频率。
■基于95％平均核苷酸同一性（ANI）和70％数字DNA-DNA杂交（dDDH）的阈值，我们将37个分离株分为6个已知物种，都属于Smc。在这项研究中测序的其余18个分离株被分配给6个新的基因组物种。在55个分离株中，我们确定了44种不同的序列类型(ST)，包括22个已知的和22个新的等位基因组合。Smc对甲氧苄啶-磺胺甲恶唑(TMP/SMX)的耐药率为3.6%,在这些分离物中检测到sul1和一类整合子整合酶基因（intI）。所有Smc分离株都对米诺环素敏感。此外，所有Smc菌株都带有mota，pilu,smf-1和Stmpr2基因。同源物种1（100%，n=9),嗜麦芽窄食单胞菌(84.21%,n=19)和窄食单胞菌(71.43%，n=7)表明afaD基因的百分比较高，这也与较高的分离率有关。除了mota,pilu,smf-1和Stmpr2基因，所有嗜麦芽窄食链球菌菌株(100%)都含有entA，gspD,卡塔,和stmPr1基因，虽然所有基因物种1菌株(100%)都含有afaD，entA,gspD,和KatA基因.
■我们的研究强调了来自菌血症患者的Smc分离株的遗传多样性，揭示了22种新颖的ST类型，58个新等位基因和6个新基因组。研究发现嗜麦芽窄食链球菌和巴氏链球菌携带更多的毒力因子，强调准确菌株识别的重要性。对于对TMP/SMX耐药的患者，米诺环素是一种有前途的替代抗生素。
