Abstract：
The present study examined whether male resveratrol intake affected mitochondrial DNA copy number (mt-cn) and telomere length (TL) in blastocysts fathered by young and aged male mice. C57BL/6N male mice supplied with water or water containing 0.1 mM resveratrol were used for embryo production at 14-23 and 48-58 weeks of age. Two-cell-stage embryos were collected from the oviducts of superovulated female mice (8-15 weeks old) and cultured for 3 days until the blastocyst stage. Mt-cn and TL levels were measured by real-time polymerase chain reaction. Resveratrol intake did not affect body weight or water consumption. Resveratrol intake increased the expression levels of SIRT1 in the liver, the antioxidative ability of serum, and extended TL in the heart, whereas there was no significant difference in mt-cn in the heart or TL in sperm. The rate of blastocyst development was significantly lower in aged male mice than in younger mice, and resveratrol intake increased the total number of blastocysts derived from both young and aged males. Resveratrol intake did not affect mt-cn or TL in blastomeres of blastocyst-stage embryos derived from young mice, but significantly increased both mt-cn and TL in blastomeres of blastocysts derived from aged fathers. In conclusion, resveratrol intake increased mt-cn and TL levels in blastocysts derived from aged male mice.
摘要：
本研究检查了雄性白藜芦醇的摄入量是否会影响年轻和老年雄性小鼠的胚泡中的线粒体DNA拷贝数（mt-cn）和端粒长度（TL）。在14-23和48-58周龄时,将供应水或含有0.1mM白藜芦醇的水的C57BL/6N雄性小鼠用于胚胎生产。从超排卵雌性小鼠(8-15周龄)的输卵管收集两细胞期胚胎,并培养3天直至胚泡期。通过实时聚合酶链反应测量Mt-cn和TL水平。白藜芦醇的摄入不会影响体重或水的消耗。白藜芦醇的摄入增加了肝脏中SIRT1的表达水平，血清的抗氧化能力，并在心脏中延伸TL，而心脏中的mt-cn或精子中的TL没有显着差异。老年雄性小鼠的囊胚发育率明显低于年轻小鼠，白藜芦醇的摄入量增加了年轻和老年男性的胚泡总数。白藜芦醇的摄入不会影响幼鼠胚泡期胚胎卵裂球的mt-cn或TL，但显着增加了来自老年父亲的胚泡卵裂球中的mt-cn和TL。总之,白藜芦醇的摄入增加了来自老年雄性小鼠的胚泡中的mt-cn和TL水平。
