Abstract：
Doxorubicin (DOX), an anthracycline group antibiotic, has been extensively employed as a potent chemotherapeutic agent for treating solid and hematopoietic tumors in humans. Amid exposure to diverse stress conditions, living organisms swiftly initiate the synthesis of heat shock proteins (HSPs), a set of highly conserved proteins. Tannic acid (TA) has garnered increasing study attention due to its special chemical properties, health benefits, and wide availability. This study\'s primary aim is to elucidate the impact of DOX and TA on the expression levels of Hsp90aa1, Hspa1a, Hspa4, and Hspa5 in the spleen tissues of rats. Sprague Dawley rats (Rattus norvegicus, male, 9-10 weeks old, 180 ± 20 g) were randomly divided into 4 groups: control, DOX (30 mg/kg cumulative), TA (50 mg/kg), and DOX + TA (5 mg/kg and 50 mg/kg, respectively). Subsequently, spleen tissues were collected from rats, and complementary DNA libraries were generated after the application process. The quantitative real-time PCR method was used to detect and quantify the mRNA expression changes of the Hsp90aa1, Hspa1a, Hspa4, and Hspa5 genes our results showed that the mRNA expressions of the targeted genes were up-regulated in rat spleen tissues exposed to DOX. However, this increase was remarkably suppressed by TA treatment. These findings suggest that TA may serve as a protective agent, mitigating the toxic effects of DOX in the rat spleen.
摘要：
阿霉素（DOX），蒽环类抗生素，已被广泛用作治疗人类实体和造血肿瘤的有效化学治疗剂。在暴露于不同压力条件下，生物体迅速启动热休克蛋白（HSPs）的合成，一组高度保守的蛋白质。单宁酸(TA)因其特殊的化学性质而受到越来越多的研究关注,健康益处,和广泛的可用性。本研究的主要目的是阐明DOX和TA对Hsp90aa1、Hspa1a表达水平的影响。年夜鼠脾组织中Hspa4、Hspa5。SpragueDawley大鼠（Rattusnorvegicus，男性,9-10周大,180±20g）随机分为4组：对照组，DOX（30mg/kg累积），TA（50mg/kg），和DOX+TA（5mg/kg和50mg/kg，分别)。随后，收集大鼠脾组织,和互补DNA文库在应用过程后产生。采用实时定量PCR方法检测并定量检测Hsp90aa1、Hspa1a、我们的结果表明，在暴露于DOX的大鼠脾组织中，Hspa4和Hspa5基因的mRNA表达上调。然而,TA处理显著抑制了这种增加。这些发现表明TA可以作为保护剂，减轻DOX对大鼠脾脏的毒性作用。
