Abstract：
The aim of this study was to assess the effect of lyophilized freezing extenders, which can be stored at room temperature, on stallion post-thaw sperm total motility (TM). Ejaculates of 28 stallions were frozen with four different extenders: two commercial freezing extenders offered worldwide and two novel lyophilized extenders (STAR and MX3), and two different cryopreservation protocols (CP1 with an equilibration period of 20 min. and CP2 with an equilibration period of 60 min.). The TM was assessed after thaw. Mean TM did not show significant differences between cryopreservation protocols within each extender. Mean TM was greater in samples diluted with STAR than in samples diluted with Botucrio (P ˂ 0.05), but no significant differences were observed for this variable between the other studied extenders. From all evaluated samples, twenty ejaculates showed the greatest TM when using the lyophilized extenders and the CP1. Thus, lyophilized extenders are a promising option for stallion sperm cryopreservation and have the advantage of storage and distribution at room temperature for at least one year.
摘要：
这项研究的目的是评估冻干冷冻补充剂的效果，可以在室温下储存，关于种马解冻后精子总运动性（TM）。28种马的射精用四种不同的补充剂冷冻：两种在全球范围内提供的商业冷冻补充剂和两种新型的冻干补充剂（STAR和MX3）。和两种不同的冷冻保存方案（CP1，平衡期为20分钟。和CP2，平衡时间为60分钟。).在解冻后评估TM。平均TM没有显示每个延长器内的冷冻保存方案之间的显着差异。用STAR稀释的样品中的平均TM大于用Botucrio稀释的样品中的平均TM（P<0.05），但该变量在其他研究的延伸剂之间没有观察到显著差异。从所有评估的样本中，当使用冻干的增量剂和CP1时,20次射精显示出最大的TM。因此，冻干的填充剂是种马精子冷冻保存的一个很有前途的选择，并且具有在室温下储存和分配至少一年的优势。
