Abstract：
Panax ginseng C.A. Mey., known for its medicinal and dietary supplement properties, primarily contains pharmacologically active ginsenosides. However, the regulatory mechanisms linking ginseng root development with ginsenoside biosynthesis are still unclear. Root meristem growth factors (RGFs) are crucial for regulating plant root growth. In our study, we identified five ginseng RGF peptide sequences from the ginseng genome and transcriptome libraries. We treated Arabidopsis and ginseng adventitious roots with exogenous Panax ginseng RGFs (PgRGFs) to assess their activities. Our results demonstrate that PgRGF1 influences gravitropic responses and reduces lateral root formation in Arabidopsis. PgRGF1 has been found to restrict the number and length of ginseng adventitious root branches in ginseng. Given the medicinal properties of ginseng, We determined the ginsenoside content and performed transcriptomic analysis of PgRGF1-treated ginseng adventitious roots. Specifically, the total ginsenoside content in ginseng adventitious roots decreased by 19.98 % and 63.71 % following treatments with 1 μM and 10 μM PgRGF1, respectively, compared to the control. The results revealed that PgRGF1 affects the accumulation of ginsenosides by regulating the expression of genes associated with auxin transportation and ginsenoside biosynthesis. These findings suggest that PgRGF1, as a peptide hormone regulator in ginseng, can modulate adventitious root growth and ginsenoside accumulation.
摘要：
人参C.A.Mey.,以其药用和膳食补充剂特性而闻名，主要含有药理活性人参皂苷。然而,人参根发育与人参皂苷生物合成的调控机制尚不清楚。根分生组织生长因子（RGF）对于调节植物根的生长至关重要。在我们的研究中,我们从人参基因组和转录组文库中鉴定了5个人参RGF肽序列。我们用外源人参RGFs（PgRGFs）处理拟南芥和人参不定根，以评估其活性。我们的结果表明，PgRGF1影响拟南芥的重力响应并减少侧根形成。已发现PgRGF1限制了人参中不定根分支的数量和长度。鉴于人参的药用特性，我们确定了人参皂苷含量，并对PgRGF1处理的人参不定根进行了转录组学分析。具体来说,用1μM和10μMPgRGF1处理后，人参不定根中人参皂苷的总含量分别下降了19.98％和63.71％，与对照相比。结果表明，PgRGF1通过调节生长素转运和人参皂苷生物合成相关基因的表达，影响人参皂苷的积累。这些发现表明，PgRGF1作为人参中的肽类激素调节剂，可以调节不定根的生长和人参皂苷的积累。
