Abstract：
In recent years, loop-mediated isothermal amplification (LAMP) has gained popularity for detecting various pathogen-specific genes due to its superior sensitivity and specificity compared to conventional polymerase chain reaction (PCR). The simplicity and flexibility of naked-eye detection of the amplicon make LAMP an ideal rapid and straightforward diagnostic tool, especially in resource-limited laboratories. Colorimetric detection is one of the simplest and most straightforward among all detection methods. This review will explore various colorimetric dyes used in LAMP techniques, examining their reaction mechanisms, advantages, limitations and latest applications.
摘要：
近年来,环介导等温扩增（LAMP）由于其与常规聚合酶链反应（PCR）相比具有更高的灵敏度和特异性，因此在检测各种病原体特异性基因方面越来越受欢迎。扩增子裸眼检测的简单性和灵活性使LAMP成为一种理想的快速和直接的诊断工具，特别是在资源有限的实验室。比色检测是所有检测方法中最简单和最直接的方法之一。这篇综述将探讨LAMP技术中使用的各种比色染料，检查他们的反应机制，优势,限制和最新的应用。
