Abstract：
Exposure to stressors has profound effects on sleep that have been linked to serotonin (5-HT) neurons of the dorsal raphe nucleus (DR). However, the DR also comprises glutamatergic neurons expressing vesicular glutamate transporter type 3 (DRVGLUT3), leading us to examine their role. Cell-type-specific tracing revealed that DRVGLUT3 neurons project to brain areas regulating arousal and stress. We found that chemogenetic activation of DRVGLUT3 neurons mimics stress-induced sleep perturbations. Furthermore, deleting VGLUT3 in the DR attenuated stress-induced sleep perturbations, especially after social defeat stress. In the DR, VGLUT3 is found in subsets of 5-HT and non-5-HT neurons. We observed that both populations are activated by acute stress, including those projecting to the ventral tegmental area. However, deleting VGLUT3 in 5-HT neurons minimally affected sleep regulation. These findings suggest that VGLUT3 expression in the DR drives stress-induced sleep perturbations, possibly involving non-5-HT DRVGLUT3 neurons.
摘要：
暴露于压力对睡眠有深远的影响，这与背中核（DR）的5-羟色胺（5-HT）神经元有关。然而,DR还包含表达3型囊泡谷氨酸转运蛋白（DRVGLUT3）的谷氨酸能神经元，引导我们审视他们的角色。细胞类型特异性追踪显示，DRVGLUT3神经元投射到调节唤醒和压力的大脑区域。我们发现DRVGLUT3神经元的化学遗传激活模拟应激诱导的睡眠扰动。此外，在DR中删除VGLUT3衰减压力诱发的睡眠扰动，尤其是在社会失败压力之后。在DR中,VGLUT3存在于5-HT和非5-HT神经元的子集中。我们观察到这两个群体都被急性压力激活，包括那些突出到腹侧被盖区的.然而,在5-HT神经元中删除VGLUT3对睡眠调节的影响最小。这些发现表明DR中的VGLUT3表达驱动应激诱导的睡眠扰动,可能涉及非5-HTDRVGLUT3神经元。
