Abstract：
Mazzaella, a genus with no genomic resources available, has extensive distribution in the cold waters of the Pacific, where they represent ecologically and economically important species. In this study, we aimed to sequence, assemble, and annotate the complete mitochondrial and chloroplast genomes from two Mazzaella spp. and characterize the intraspecific variation among them. We report for the first time seven whole organellar genomes (mitochondria: OR915856, OR947465, OR947466, OR947467, OR947468, OR947469, OR947470; chloroplast: OR881974, OR909680, OR909681, OR909682, OR909683, OR909684, OR909685) obtained through high-throughput sequencing for six M. laminarioides sampled from three Chilean regions and one M. membranacea. Sequenced Mazzaella mitogenomes have identical gene number, gene order, and genome structure. The same results were observed for assembled plastomes. A total of 52 genes were identified in mitogenomes, and a total of 235 genes were identified in plastomes. Although the M. membranacea plastome included a full-length pbsA gene, in all M. laminarioides samples, the pbsA gene was split in three open reading frames (ORFs). Within M. laminarioides, we observed important plastome lineage-specific variations, such as the pseudogenization of the two hypothetical protein-coding genes, ycf23 and ycf45. Nonsense mutations in the ycf23 and ycf45 genes were only detected in the northern lineage. These results are consistent with phylogenetic reconstructions and divergence time estimation using concatenated coding sequences that not only support the monophyly of M. laminarioides but also underscore that the three M. laminarioides lineages are in an advanced stage of divergence. These new results open the question of the existence of still undisclosed species in M. laminarioides.
摘要：
Mazzaella,一个没有基因组资源的属，在太平洋的寒冷水域有广泛的分布，它们代表着生态和经济上重要的物种。在这项研究中,我们的目标是序列，组装,并注释来自两个Mazzaellaspp的完整线粒体和叶绿体基因组。并表征它们之间的种内变异。我们首次报告了七个完整的细胞器基因组（线粒体：OR915856，OR947465，OR947466，OR947467，OR947468，OR947469，OR947470；叶绿体：OR881974，OR909680，OR909681，OR909682，OR909683，OR909684，OR909684，通过三个高通量M。测序的马扎氏菌有丝分裂基因组具有相同的基因编号，基因顺序，和基因组结构。对于组装的质体观察到相同的结果。共有52个基因在有丝分裂基因组中被鉴定出来,在质体中总共鉴定出235个基因。虽然膜孢霉体包含一个全长的pbsA基因,在所有Laminarioides样本中，pbsA基因被分成三个开放阅读框(ORF)。在拉米诺德内，我们观察到重要的质体谱系特异性变异，例如两个假设的蛋白质编码基因的伪生成，ycf23和ycf45。仅在北方谱系中检测到ycf23和ycf45基因中的无义突变。这些结果与使用串联编码序列的系统发育重建和发散时间估计一致，这些序列不仅支持M.laminarioides的单系，而且还强调了三个M.laminarioides谱系处于发散的高级阶段。这些新的结果打开了一个问题，即laminarioides中仍存在未公开的物种。
