Abstract：
Antibiotics\' usefulness is threatened by multi-drugs resistance in harmful microorganisms because of abuse and regulatory problems. Emerging microbes, resistance mechanisms and antimicrobial drugs all require extensive investigation. Evaluation of the in vitro antibacterial activity of Methanolic extracts isolated from Black pepper seeds (Piper nigrum L.) against two infection causing pathogens, Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. From July 2022 and June 2023, this experimental study was conducted at the Mymensingh Medical College\'s Department of Pharmacology and Therapeutics in conjunction with the Department of Microbiology. The solvents Methanol and 10.0% Di-Methyl Sulfoxide (DMSO) were used to make the extract. Using the disc diffusion and broth dilution methods, the antibacterial activity of methanolic extract of black pepper seeds (MBPE) was evaluated at various doses. Using the broth dilution procedure, the conventional antibiotic Ciprofloxacin was utilized, and the outcome was contrasted with that of Methanol extracts. Methanolic extract of black pepper seeds (MBPE) at seven distinct concentrations (100, 80, 60, 40, 20, 10 and 5mg/ml) were utilized, then later in chosen concentrations as needed to confirm the extracts\' more precise margin of antimicrobial sensitivity. At 80mg/ml and above doses of the MBPE, it had an inhibitory impact against the aforementioned microorganisms. For Staphylococcus aureus and Pseudomonas aeruginosa the MIC were 60 and 70mg/ml in MBPE respectively. As of the MIC of Ciprofloxacin was 1μg/ml against Staphylococcus aureus and 1.5μg/ml for Pseudomonas aeruginosa. In comparison to MICs of MBPE for the test organisms, the MIC of Ciprofloxacin was the lowest. This study clearly shows that Staphylococcus aureus and Pseudomonas aeruginosa are sensitive to the methanolic extract of black pepper seeds\' antibacterial properties.
摘要：
由于滥用和监管问题，有害微生物中的多药耐药性威胁着抗生素的有效性。新兴的微生物，耐药机制和抗菌药物都需要广泛的研究。评估从黑胡椒种子（PipernigrumL.）中分离出的甲醇提取物对两种引起感染的病原体的体外抗菌活性，革兰阳性金黄色葡萄球菌和革兰阴性铜绿假单胞菌。从2022年7月到2023年6月，这项实验研究是在Mymensingh医学院的药理学和治疗学系与微生物学系一起进行的。溶剂甲醇和10.0%二甲亚砜(DMSO)用于制备提取物。使用圆盘扩散和肉汤稀释方法，在各种剂量下评估黑胡椒种子的甲醇提取物（MBPE）的抗菌活性。使用肉汤稀释程序，使用了常规抗生素环丙沙星，结果与甲醇提取物的结果形成对比。使用七个不同浓度（100、80、60、40、20、10和5mg/ml）的黑胡椒种子（MBPE）的甲醇提取物，然后根据需要选择浓度以确认提取物对抗菌药物敏感性的更精确范围。在80mg/ml及以上剂量的MBPE下，它对上述微生物有抑制作用。对于金黄色葡萄球菌和铜绿假单胞菌,在MBPE中的MIC分别为60和70mg/ml。环丙沙星对金黄色葡萄球菌的MIC为1μg/ml,对铜绿假单胞菌的MIC为1.5μg/ml。与测试生物的MBPEMIC相比，环丙沙星的MIC最低。这项研究清楚地表明，金黄色葡萄球菌和铜绿假单胞菌对黑胡椒种子的甲醇提取物的抗菌性能敏感。
