Abstract：
Microorganisms form dynamic communities with plants, providing benefits such as nutrient acquisition and stress resilience. Understanding how these microorganisms are affected by environmental factors such as growth conditions and soil characteristics are essential for harnessing these communities for sustainable agriculture practices and their response to climate change. The microbiome associated to Lupinus angustifolius, a legume native in Europe, with a high protein value and stress resilience was characterized for the first time. Using 16S rRNA gene and ITS amplicon sequencing, we characterized the compositional and temporal changes of the bacterial and fungal communities associated to the soil, rhizosphere, and plant compartments where Lupinus angustifolius grows naturally. Our results suggest that the main difference in the soil microbial communities is related to the edaphic properties, although environmental factors such as temperature, humidity or rainfall also influenced the composition of the soil microbial communities. We also characterized the bacterial communities associated with the rhizosphere, roots, nodules, and leaves of wild plants collected in the field and compared them against plants obtained under greenhouse conditions. In the plant compartments, the bacterial composition appeared to be more affected by the growing conditions (field vs greenhouse), than by soil characteristics or location. These results can be used to identify key taxa that may play crucial roles in the development and adaptation of the host plant and its associated microbiota to environmental changes and highlight the importance of characterizing the plant microbiomes in their natural habitats. Soil, influenced by climatic seasons, shapes the plant microbiome assembly. Lupinus recruits a core microbiome across rhizosphere, roots, nodules, and leaves, that is stable across locations. However, cultivation conditions may alter microbiome dynamics, impacting the adaptability of its components. Wild plants show a resilient and adaptable microbiome while germination and cultivation in greenhouse conditions alter its composition and vulnerability.
摘要：
微生物与植物形成动态群落，提供营养获取和压力弹性等益处。了解这些微生物如何受到环境因素的影响，如生长条件和土壤特性，对于利用这些社区进行可持续农业实践及其对气候变化的反应至关重要。与羽扇豆相关的微生物组，一个原产于欧洲的豆科植物,具有较高的蛋白质值和压力韧性首次被表征。使用16SrRNA基因和ITS扩增子测序，我们描述了与土壤相关的细菌和真菌群落的组成和时间变化，根际,和羽扇豆自然生长的植物隔间。我们的结果表明，土壤微生物群落的主要差异与土壤性质有关，虽然环境因素如温度，湿度或降雨也会影响土壤微生物群落的组成。我们还描述了与根际相关的细菌群落，根,结节,和在田间收集的野生植物的叶子，并将它们与在温室条件下获得的植物进行比较。在植物隔间里，细菌成分似乎更受生长条件的影响（田间与温室），而不是土壤特性或位置。这些结果可用于确定关键分类群，这些分类群可能在寄主植物及其相关微生物群的发育和适应环境变化中起关键作用，并强调了在其自然栖息地中表征植物微生物群的重要性。土壤,受气候季节的影响，塑造植物微生物组组装。羽扇豆在根际招募核心微生物组，根,结节,和叶子，在不同地点是稳定的。然而,培养条件可能会改变微生物组动态，影响其组件的适应性。野生植物表现出弹性和适应性的微生物组，而在温室条件下的发芽和栽培会改变其组成和脆弱性。
