Abstract：
Lymphoma is the most common tumour of domestic cats, developing most frequently in the small intestine. Feline small intestinal lymphoma predominantly demonstrates a T-cell immunophenotype identified by standard immunopositivity for T cells with CD3 or immunopositivity for B cells with CD20. In contrast, a wide spectrum of immunohistochemical antibodies are applied in humans to diagnose the various specific lymphoma subtypes according to the WHO classification. Our aim was to augment our knowledge of immunophenotypes in feline non-B-cell lymphomas forming macroscopic masses in the intestinal tract. We evaluated the combined immunohistochemistry and flow cytometry findings from 15 cases. Neoplastic lymphoid cells were immunopositive for CD3 in 93% (14/15), granzyme B in 87% (13/15), CD5 in 20% (3/15), CD8 in 13% (2/15), CD4 in 7% (1/15) and CD56 in 7% (1/15) of cases. Cytotoxic granules indicating a cytotoxic origin of the neoplastic cells were identified by histopathology only in 13% (2/15) and by cytology in 47% (7/15) of the cases. Without immunohistochemical labelling of the cytotoxic protein granzyme B, the cytotoxic status would have been missed in 46% (6/13) of the cytological and in 85% (11/13) of the histopathological slides. These findings suggest that more complex immunophenotyping may advance our understanding and help prognosticate small intestinal T-cell lymphoma in cats.
摘要：
淋巴瘤是家猫最常见的肿瘤，最常发生在小肠。猫小肠淋巴瘤主要显示通过具有CD3的T细胞的标准免疫阳性或具有CD20的B细胞的标准免疫阳性鉴定的T细胞免疫表型。相比之下,根据WHO分类，在人类中应用了广泛的免疫组织化学抗体来诊断各种特定的淋巴瘤亚型。我们的目的是增加我们对在肠道中形成宏观肿块的猫科动物非B细胞淋巴瘤的免疫表型的了解。我们评估了15例患者的免疫组织化学和流式细胞术联合发现。93％（14/15）的肿瘤淋巴样细胞对CD3免疫阳性，颗粒酶B占87%(13/15)，CD5在20%（3/15），13％的CD8（2/15），7%(1/15)的CD4和7%(1/15)的CD56病例。仅在13％（2/15）的病例中通过组织病理学和在47％（7/15）的病例中通过细胞学鉴定了表明肿瘤细胞的细胞毒性起源的细胞毒性颗粒。没有细胞毒性蛋白颗粒酶B的免疫组织化学标记，在46%(6/13)的细胞学切片和85%(11/13)的组织病理学切片中,细胞毒性状态会被遗漏.这些发现表明，更复杂的免疫表型可能会增进我们的理解，并有助于预测猫的小肠T细胞淋巴瘤。
