Abstract：
Copper oxide nanosheets (CuO NSs) have been successfully obtained by exploiting an effective one-step approach of sugar-blowing method followed by calcination. The nanosheets were characterized by several techniques like X-ray powder diffraction (XRD), Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Impressively, CuO NSs display haloperoxidase (HPO) like catalytic activity which catalyses the oxidation of chloride ions by H2O2 giving rise to reactive chlorine species (RCS). A sensitive and selective colorimetric sensor was then demonstrated via the oxidation of chromogenic substrate 3,3\',5,5\'- tetramethylbenzidine (TMB) by the novel nanoenzyme CuO NSs through the generation of RCS for H2O2 and glucose detection with limit of detection of 109 nM and 21 nM in the linear ranges of 4.6 µM to 769 µM and 0.22 µM to 19.57 µM respectively. Additionally, the methodology is validated for the analysis of glucose in real samples.
摘要：
氧化铜纳米片（CuONSs）已成功地通过利用一种有效的一步方法的制糖方法，然后煅烧获得。通过X射线粉末衍射（XRD）等几种技术对纳米片进行了表征，透射电子显微镜(TEM),X射线光电子能谱(XPS)和傅里叶变换红外光谱(FTIR)。令人印象深刻的是,CuONSs表现出类似卤过氧化物酶（HPO）的催化活性，可催化H2O2对氯离子的氧化，从而产生反应性氯物种（RCS）。然后通过显色底物3,3'的氧化证明了灵敏和选择性的比色传感器，5,5'-四甲基联苯胺（TMB）通过新型纳米酶CuONSs产生用于H2O2和葡萄糖检测的RCS，检出限为109nM和21nM，线性范围分别为4.6µM至769µM和0.22µM至19.57µM。此外,该方法在实际样品中的葡萄糖分析中得到了验证。
