PDF(Pubmed)
Abstract：
Placental hypoxia is hazardous to maternal health as well as fetal growth and development. Preeclampsia and intrauterine growth restriction are common pregnancy problems, and one of the causes is placental hypoxia. Placental hypoxia is linked to a number of pregnancy illnessesv. To investigate their potential function in anoxic circumstances, we mimicked the anoxic environment of HTR-8/Svneo cells and performed lncRNA and circRNA studies on anoxic HTR-8/Svneo cells using high-throughput RNA sequencing. The miRNA target genes were predicted by integrating the aberrant expression of miRNAs in the placenta of preeclampsia and intrauterine growth restriction, and a ceRNA network map was developed to conduct a complete transcriptomic and bioinformatics investigation of circRNAs and lncRNAs. The signaling pathways in which the genes were primarily engaged were predicted using GO and KEGG analyses. To propose a novel explanation for trophoblastic organism failure caused by lncRNAs and circRNAs in an anoxic environment.
摘要：
胎盘缺氧对母体健康和胎儿生长发育都有危害。子痫前期和宫内生长受限是常见的妊娠问题,原因之一是胎盘缺氧。胎盘缺氧与许多妊娠疾病有关。为了研究它们在缺氧环境下的潜在功能，我们模拟了HTR-8/Svneo细胞的缺氧环境,并使用高通量RNA测序对缺氧HTR-8/Svneo细胞进行了lncRNA和circRNA研究.通过整合子痫前期和宫内生长受限胎盘中miRNA的异常表达来预测miRNA靶基因，并开发了ceRNA网络图以对circRNAs和lncRNAs进行完整的转录组学和生物信息学研究。使用GO和KEGG分析预测基因主要参与的信号传导途径。为缺氧环境中lncRNAs和circRNAs引起的滋养细胞衰竭提出新的解释。
