PDF(Pubmed)
Abstract：
BACKGROUND: Tissue conditioners are used for treating and improving the tissues supporting complete dentures. On the other hand, recent advances in nanotechnology have revolutionized various fields of science, including dentistry. The present study aimed to investigate novel antimicrobial applications of copper oxide nanoparticle-based tissue conditioner used in complete prostheses.
METHODS: The present experimental study included 126 tissue conditioner samples with different concentrations of copper oxide nanoparticles (20%, 10%, 5%, 2.5%, 1.25%, 0.625%, and 0% w/w). The samples were incubated with Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans in 24-well plates for 24 h. Then, samples from the wells were re-incubated for 24 h, and the microorganisms were counted.
RESULTS: The culture media containing E. faecalis and P. aeruginosa showed significantly different growth between different nanoparticle concentrations following 24 h (P < 0.001), showing a reduction in bacterial growth with increased nanoparticle concentration. Both bacteria did not show any growth at the 20% concentration. However, C. albicans showed significant differences in growth between different nanoparticle concentrations following 48 h (P < 0.001), showing a reduction in growth with increased nanoparticle concentration. Also, the least growth was observed at the 20% concentration.
CONCLUSIONS: In conclusion, the CuO nanoparticles were prepared using a green synthesis methon in the suitable sizes. Moreover, the tissue conditioners containing CuO nanoparticles showed acceptable antimicrobial properties against E. faecalis, P. aeruginosa, and C. albicans.
METHODS: The present experimental study included 126 tissue conditioner samples with different concentrations of copper oxide nanoparticles (20%, 10%, 5%, 2.5%, 1.25%, 0.625%, and 0% w/w). The samples were incubated with Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans in 24-well plates for 24 h. Then, samples from the wells were re-incubated for 24 h, and the microorganisms were counted.
RESULTS: The culture media containing E. faecalis and P. aeruginosa showed significantly different growth between different nanoparticle concentrations following 24 h (P < 0.001), showing a reduction in bacterial growth with increased nanoparticle concentration. Both bacteria did not show any growth at the 20% concentration. However, C. albicans showed significant differences in growth between different nanoparticle concentrations following 48 h (P < 0.001), showing a reduction in growth with increased nanoparticle concentration. Also, the least growth was observed at the 20% concentration.
CONCLUSIONS: In conclusion, the CuO nanoparticles were prepared using a green synthesis methon in the suitable sizes. Moreover, the tissue conditioners containing CuO nanoparticles showed acceptable antimicrobial properties against E. faecalis, P. aeruginosa, and C. albicans.
摘要：
背景：组织调理剂用于治疗和改善支持全口义齿的组织。另一方面,纳米技术的最新进展彻底改变了科学的各个领域，包括牙科。本研究旨在研究用于完整假体的基于氧化铜纳米颗粒的组织调理剂的新型抗菌应用。
方法：本实验研究包括126个组织调理剂样品，其中含有不同浓度的氧化铜纳米颗粒（20％，10%,5%,2.5%,1.25%,0.625%，和0%w/w)。将样品与粪肠球菌一起孵育，铜绿假单胞菌,和白色念珠菌在24孔板中放置24小时。然后，将来自孔的样品重新孵育24小时，并对微生物进行计数。
结果：含有粪肠球菌和铜绿假单胞菌的培养基在24小时后显示出不同纳米颗粒浓度之间的显着生长差异（P<0.001），显示随着纳米颗粒浓度的增加，细菌生长减少。两种细菌在20%浓度下均未显示任何生长。然而,48h后，白色念珠菌在不同纳米颗粒浓度之间的生长表现出显著差异(P<0.001)，显示随着纳米粒子浓度的增加，生长减少。此外，在20%浓度下观察到最小的生长。
结论：结论：CuO纳米粒子是使用绿色合成方法以合适的尺寸制备的。此外，含有CuO纳米颗粒的组织调理剂对粪肠球菌显示出可接受的抗菌性能，铜绿假单胞菌,还有白色念珠菌.
方法：本实验研究包括126个组织调理剂样品，其中含有不同浓度的氧化铜纳米颗粒（20％，10%,5%,2.5%,1.25%,0.625%，和0%w/w)。将样品与粪肠球菌一起孵育，铜绿假单胞菌,和白色念珠菌在24孔板中放置24小时。然后，将来自孔的样品重新孵育24小时，并对微生物进行计数。
结果：含有粪肠球菌和铜绿假单胞菌的培养基在24小时后显示出不同纳米颗粒浓度之间的显着生长差异（P<0.001），显示随着纳米颗粒浓度的增加，细菌生长减少。两种细菌在20%浓度下均未显示任何生长。然而,48h后，白色念珠菌在不同纳米颗粒浓度之间的生长表现出显著差异(P<0.001)，显示随着纳米粒子浓度的增加，生长减少。此外，在20%浓度下观察到最小的生长。
结论：结论：CuO纳米粒子是使用绿色合成方法以合适的尺寸制备的。此外，含有CuO纳米颗粒的组织调理剂对粪肠球菌显示出可接受的抗菌性能，铜绿假单胞菌,还有白色念珠菌.
