Abstract：
Amauroderma rugosum (AR) is commonly recognized as a medicinal fungus, often used as an alternative to Ganoderma lucidum. There is a scarcity of comprehensive and in-depth research on its bioactive polysaccharides and their associated biological activities. Herein, we isolated the polysaccharide fractions extracted from AR (ARPs) and investigated their primary structure and anti-angiogenic activities, given that various diseases are associated with excessive angiogenesis. Four polysaccharide fractions including ARP-0, ARP-1, ARP-2, and ARP-5 were heteropolysaccharides with different molecular weights, monosaccharide compositions, and micromorphologies, highlighting their varying bioactive profiles. Treatment of human umbilical vein endothelial cells with these polysaccharide fractions showed that only ARP-5 inhibited cell proliferation after vascular endothelial growth factor (VEGF) stimulation. Similarly, ARP-5 inhibited human umbilical vein endothelial cells migration, invasion, and tube formation upon VEGF (50 ng/mL) treatment. Moreover, compared with the insignificant effects of ARP-0, ARP-1, and ARP-2, ARP-5 impeded angiogenesis in zebrafish embryos. Additionally, ARP-5 downregulated the VEGF/VEGFR2 signaling pathway in a dose-dependent manner, suggesting that ARP-5 exerts its anti-angiogenic activities by blocking the VEGF/VEGFR2-mediated angiogenesis signaling pathway. Taken together, the study findings shed light on the primary structure and bioactivity of ARPs.
摘要：
大黄鱼（AR）通常被认为是一种药用真菌，通常用作灵芝的替代品。目前对其生物活性多糖及其相关生物活性的全面、深入的研究还很少。在这里，我们分离了从AR（ARPs）中提取的多糖部分，并研究了它们的主要结构和抗血管生成活性，鉴于各种疾病与过度血管生成有关。4种多糖组分包括ARP-0、ARP-1、ARP-2和ARP-5是不同分子量的杂多糖,单糖组合物，和微观形态，突出它们不同的生物活性特征。用这些多糖级分处理人脐静脉内皮细胞表明，在血管内皮生长因子（VEGF）刺激后，只有ARP-5抑制细胞增殖。同样，ARP-5抑制人脐静脉内皮细胞迁移,入侵,和VEGF(50ng/mL)处理后的管形成。此外，与ARP-0，ARP-1和ARP-2的作用不明显相比，ARP-5阻碍了斑马鱼胚胎中的血管生成。此外,ARP-5以剂量依赖性方式下调VEGF/VEGFR2信号通路,提示ARP-5通过阻断VEGF/VEGFR2介导的血管生成信号通路发挥其抗血管生成活性。一起来看,研究结果揭示了ARPs的主要结构和生物活性。
