Abstract：
In India, fish roes are generally considered worthless garbage and disposed of without recovering the valuable molecules, creating environmental and disposal problems. The present investigation aimed to optimize the extraction conditions, partial purification, and characterization of sialoglycoproteins (RRSGP) from Labeo rohita (rohu) roes. RSM generated optimum conditions for maximum RRSGP (70.49 %) extraction, which were 1.25 M NaCl, 1:32.5(w/v) solid-to-liquid ratio, 47.5 °C temperature, and 3 h time. Further, sialoglycoproteins from RRSGPs were partially purified, and result revealed that obtained peak-1 (PRRSGP) using QFF anion exchange chromatography exhibited higher glycoprotein and sialic acid content (p < 0.05). SDS-PAGE pattern of PRRSGP presented dominant bands of 97 kDa and 27 kDa glycoproteins. FTIR spectrum of PRRSGP confirmed the presence of glycated proteins. HPLC analysis revealed that PRRSGP consists of Neu5Ac. Furthermore, β-elimination reaction elucidated that PRRSGP contained N-glycosidic linkage. PRRSGP exhibited tyrosine and glutamate as primary amino acids. Glycan part of PRRSGP presented mannose and N-acetyl galactosamine as dominant neutral and amino sugar, respectively. Furthermore, PRRSGP exhibited antioxidant activity with EC50 value for DPPH (8.79 mg/ml) and ABTS (2.21 mg/ml). Besides, RRSGP displayed better protein solubility, foaming, and emulsion properties. Therefore, rohu roes are potential source of sialoglycoproteins that can be recovered and used as bio-functional ingredients in food and nutraceutical applications.
摘要：
在印度,鱼卵通常被认为是毫无价值的垃圾，在没有回收有价值的分子的情况下被处理掉，造成环境和处置问题。本研究旨在优化提取条件，部分纯化,和来自Labeorohita（rohu）卵的唾液酸糖蛋白（RRSGP）的表征。RSM为最大RRSGP(70.49%)提取产生了最佳条件,是1.25MNaCl，1:32.5(w/v)固液比,温度为47.5°C,和3小时。此外，来自RRSGP的唾液酸糖蛋白被部分纯化，结果表明，使用QFF阴离子交换色谱获得的峰-1（PRRSGP）表现出更高的糖蛋白和唾液酸含量（p<0.05）。PRRSGP的SDS-PAGE图谱呈现了97kDa和27kDa糖蛋白的显性条带。PRRSGP的FTIR光谱证实存在糖化蛋白。HPLC分析显示PRRSGP由Neu5Ac组成。此外，β-消除反应阐明PRRSGP含有N-糖苷键。PRRSGP显示酪氨酸和谷氨酸作为一级氨基酸。PRRSGP的聚糖部分呈现甘露糖和N-乙酰半乳糖胺作为主要的中性和氨基糖，分别。此外，PRRSGP表现出抗氧化活性，对DPPH（8.79mg/ml）和ABTS（2.21mg/ml）具有EC50值。此外,RRSGP显示出更好的蛋白质溶解度，发泡,和乳液性能。因此,罗湖鱼卵是唾液酸糖蛋白的潜在来源，可以回收并用作食品和营养食品应用中的生物功能成分。
