METHODS: 192 patients with either polyps or CRC at varying stages were recruited from May 2017 to December 2020. Real-time PCR assessed infection rates and bacterial loads in CRC tissues.
RESULTS: B. fragilis infection was notably higher in CRC tissues (51.6 %) than polyps (9.4 %), with a fivefold higher relative load. Positive associations were found in stages II and III, indicating a fivefold increase in CRC progression risk. F. nucleatum infection rates were significantly higher in CRC tissues (55.2 %) than in polyps (10.5 %). In stage II, the infection rate exceeded that in adjacent tissues. The relative load of F. nucleatum was higher in stage III than in stages I and II. Positive F. nucleatum patients had a 3.2 times higher risk of CRC progression.
CONCLUSIONS: These findings suggest associations between loading of F. nucleatum or/and B. fragilis with the advanced stages of CRC.
方法:从2017年5月至2020年12月招募了192名不同阶段的息肉或CRC患者。实时PCR评估CRC组织中的感染率和细菌负荷。
结果:B.CRC组织中的脆弱感染(51.6%)明显高于息肉(9.4%),相对负荷高五倍。在第二阶段和第三阶段发现了正相关,表明CRC进展风险增加五倍。CRC组织中F.核仁感染率(55.2%)明显高于息肉(10.5%)。在第二阶段,感染率超过邻近组织。第III阶段F.核仁的相对负荷高于第I和II阶段。核仁F.阳性患者的CRC进展风险高3.2倍。
结论:这些发现提示核仁F.或/和脆弱芽孢杆菌的负荷与CRC的晚期之间存在关联。