Abstract：
BACKGROUND: Colorectal cancer (CRC) is a significant global health concern, and understanding the role of specific bacterial infections in its development and progression is of increasing interest. This cross-sectional study investigated the associations between Bacteroides fragilis (B. fragilis) and Fusobacterium nucleatum (F. nucleatum) infections and Vietnamese CRC patients.
METHODS: 192 patients with either polyps or CRC at varying stages were recruited from May 2017 to December 2020. Real-time PCR assessed infection rates and bacterial loads in CRC tissues.
RESULTS: B. fragilis infection was notably higher in CRC tissues (51.6 %) than polyps (9.4 %), with a fivefold higher relative load. Positive associations were found in stages II and III, indicating a fivefold increase in CRC progression risk. F. nucleatum infection rates were significantly higher in CRC tissues (55.2 %) than in polyps (10.5 %). In stage II, the infection rate exceeded that in adjacent tissues. The relative load of F. nucleatum was higher in stage III than in stages I and II. Positive F. nucleatum patients had a 3.2 times higher risk of CRC progression.
CONCLUSIONS: These findings suggest associations between loading of F. nucleatum or/and B. fragilis with the advanced stages of CRC.
METHODS: 192 patients with either polyps or CRC at varying stages were recruited from May 2017 to December 2020. Real-time PCR assessed infection rates and bacterial loads in CRC tissues.
RESULTS: B. fragilis infection was notably higher in CRC tissues (51.6 %) than polyps (9.4 %), with a fivefold higher relative load. Positive associations were found in stages II and III, indicating a fivefold increase in CRC progression risk. F. nucleatum infection rates were significantly higher in CRC tissues (55.2 %) than in polyps (10.5 %). In stage II, the infection rate exceeded that in adjacent tissues. The relative load of F. nucleatum was higher in stage III than in stages I and II. Positive F. nucleatum patients had a 3.2 times higher risk of CRC progression.
CONCLUSIONS: These findings suggest associations between loading of F. nucleatum or/and B. fragilis with the advanced stages of CRC.
摘要：
背景：结直肠癌（CRC）是一个重要的全球健康问题，了解特定细菌感染在其发展和进展中的作用越来越受到人们的关注。这项横断面研究调查了脆弱拟杆菌(B.脆弱)和具核梭杆菌(F.核仁）感染和越南CRC患者。
方法：从2017年5月至2020年12月招募了192名不同阶段的息肉或CRC患者。实时PCR评估CRC组织中的感染率和细菌负荷。
结果：B.CRC组织中的脆弱感染（51.6％）明显高于息肉（9.4％），相对负荷高五倍。在第二阶段和第三阶段发现了正相关，表明CRC进展风险增加五倍。CRC组织中F.核仁感染率（55.2％）明显高于息肉（10.5％）。在第二阶段,感染率超过邻近组织。第III阶段F.核仁的相对负荷高于第I和II阶段。核仁F.阳性患者的CRC进展风险高3.2倍。
结论：这些发现提示核仁F.或/和脆弱芽孢杆菌的负荷与CRC的晚期之间存在关联。
方法：从2017年5月至2020年12月招募了192名不同阶段的息肉或CRC患者。实时PCR评估CRC组织中的感染率和细菌负荷。
结果：B.CRC组织中的脆弱感染（51.6％）明显高于息肉（9.4％），相对负荷高五倍。在第二阶段和第三阶段发现了正相关，表明CRC进展风险增加五倍。CRC组织中F.核仁感染率（55.2％）明显高于息肉（10.5％）。在第二阶段,感染率超过邻近组织。第III阶段F.核仁的相对负荷高于第I和II阶段。核仁F.阳性患者的CRC进展风险高3.2倍。
结论：这些发现提示核仁F.或/和脆弱芽孢杆菌的负荷与CRC的晚期之间存在关联。
