Abstract：
The cryopreservation of teleost eggs and embryos remains challenging, and there are no previous reports that demonstrate successful cryopreservation in medaka (Oryzias latipes). We have reported egg and sperm production, followed by the generation of donor-derived offspring by transplanting vitrified whole testes-derived testicular cells into surrogate fish. The vitrification solutions contained ethylene glycol, sucrose, and ficoll. In this study, we replaced sucrose with trehalose in the vitrification solution and medaka whole testis was vitrified with the solution. The post-vitrification survival (72.8±3.5%) was markedly improved compared with that achieved using the sucrose-containing solution (44.7±4.2%). Moreover, we demonstrated the production of eggs, sperm, and donor-derived offspring from testicular cells transplanted into surrogate recipients. The phenotype of donor-derived offspring was identical to that of transplanted testicular cells. These findings suggest that trehalose is effective for the vitrification of medaka whole testis and can be considered an effective and reliable method for the long-term preservation of their genetic resources.
摘要：
硬骨鱼卵和胚胎的冷冻保存仍然具有挑战性，并且没有以前的报道表明在medaka（Oryziaslatipes）中成功的冷冻保存。我们已经报道了卵子和精子的产生，然后通过将玻璃化的整个睾丸衍生的睾丸细胞移植到代孕鱼中，从而产生供体衍生的后代。玻璃化溶液含有乙二醇，蔗糖,还有Ficoll.在这项研究中,我们在玻璃化溶液中用海藻糖代替蔗糖，并将整个睾丸用溶液玻璃化。与使用含蔗糖的溶液（44.7±4.2％）相比，玻璃化后的存活率（72.8±3.5％）显着提高。此外，我们展示了鸡蛋的生产，精子,和来自睾丸细胞的供体后代移植到代孕受体。供体后代的表型与移植的睾丸细胞的表型相同。这些发现表明，海藻糖对medaka整个睾丸的玻璃化是有效的，可以被认为是长期保存其遗传资源的有效而可靠的方法。
