Abstract：
OBJECTIVE: To improve the current recommendations for the diagnosis of canine heartworm (Dirofilaria immitis) disease.
METHODS: Blood samples collected from 35 shelter dogs in the Republic of Korea.
METHODS: Samples were tested for the presence of microfilaria using the modified Knott (MK) test and D immitis DNA using species-specific loop-mediated isothermal amplification (LAMP) PCR. The blood samples were additionally assessed for the presence of heartworm antigens using the Antigen Rapid Canine Heartworm AG Test Kit 2.0 (Bionote Co). The performance of the MK test and LAMP PCR was assessed through statistical analysis, with a paired McNemar test utilized for comparison.
RESULTS: The heartworm antigen was detected in 28.5% of the subjects. Of the 10 positive animals, the MK test detected microfilaria in 4 of 35 (11.4%) animals, and LAMP PCR detected D immitis DNA in 6 of 35 (17.1%). The results of this study indicate that the LAMP PCR showed more positive results in samples compared to the conventional MK test.
CONCLUSIONS: The D immitis-specific LAMP PCR assay has the potential to function as an alternative to current detection methods. It could complement the existing antigen detection tests in diagnosing canine heartworm infections.
METHODS: Blood samples collected from 35 shelter dogs in the Republic of Korea.
METHODS: Samples were tested for the presence of microfilaria using the modified Knott (MK) test and D immitis DNA using species-specific loop-mediated isothermal amplification (LAMP) PCR. The blood samples were additionally assessed for the presence of heartworm antigens using the Antigen Rapid Canine Heartworm AG Test Kit 2.0 (Bionote Co). The performance of the MK test and LAMP PCR was assessed through statistical analysis, with a paired McNemar test utilized for comparison.
RESULTS: The heartworm antigen was detected in 28.5% of the subjects. Of the 10 positive animals, the MK test detected microfilaria in 4 of 35 (11.4%) animals, and LAMP PCR detected D immitis DNA in 6 of 35 (17.1%). The results of this study indicate that the LAMP PCR showed more positive results in samples compared to the conventional MK test.
CONCLUSIONS: The D immitis-specific LAMP PCR assay has the potential to function as an alternative to current detection methods. It could complement the existing antigen detection tests in diagnosing canine heartworm infections.
摘要：
目的：改进目前犬心丝虫病的诊断建议。
方法：从大韩民国的35只收容所狗身上采集血液样本。
方法：使用改良的Knott（MK）测试和使用物种特异性环介导的等温扩增（LAMP）PCR测试DimmitisDNA的存在。另外使用抗原快速犬心虫AG测试试剂盒2.0(BionoteCo)评估血液样品中是否存在心丝虫抗原。通过统计分析评估MK测试和LAMPPCR的性能，使用配对的McNemar测试进行比较。
结果：在28.5%的受试者中检测到了心丝虫抗原。在10只阳性动物中，MK测试在35只动物中的4只(11.4%)中检测到微丝带菌，LAMPPCR在35人中有6人(17.1%)检测到D基因。这项研究的结果表明，与常规MK测试相比，LAMPPCR在样品中显示出更多的阳性结果。
结论：Dimmitis特异性LAMPPCR检测有可能替代当前的检测方法。它可以补充现有的诊断犬心丝虫感染的抗原检测测试。
方法：从大韩民国的35只收容所狗身上采集血液样本。
方法：使用改良的Knott（MK）测试和使用物种特异性环介导的等温扩增（LAMP）PCR测试DimmitisDNA的存在。另外使用抗原快速犬心虫AG测试试剂盒2.0(BionoteCo)评估血液样品中是否存在心丝虫抗原。通过统计分析评估MK测试和LAMPPCR的性能，使用配对的McNemar测试进行比较。
结果：在28.5%的受试者中检测到了心丝虫抗原。在10只阳性动物中，MK测试在35只动物中的4只(11.4%)中检测到微丝带菌，LAMPPCR在35人中有6人(17.1%)检测到D基因。这项研究的结果表明，与常规MK测试相比，LAMPPCR在样品中显示出更多的阳性结果。
结论：Dimmitis特异性LAMPPCR检测有可能替代当前的检测方法。它可以补充现有的诊断犬心丝虫感染的抗原检测测试。
