Abstract：
BACKGROUND: Neonates undergo numerous painful procedures throughout their hospitalization. Repeated procedural pain may cause adverse long-term effects. Glucose as a non-pharmacological analgesia, is used for neonate pain management. In this study, potential mechanism of attenuate pain induced by glucose in neurodevelopment effect of neonate pain stimulus was investigated.
METHODS: Neonatal rats to perform a repetitive injury model and glucose intervention model in the postnatal day 0-7(P0-7). Pain thresholds were measured by von Frey test weekly. The puberty behavioral outcome, tissue loss and protein expression in hippocampus were analyzed.
RESULTS: Oral administration of glucose after repeated pain stimulation can maintain the hippocampal structure in, and reduce the expressions of corticotropin releasing factor (CFR) and glucocorticoid receptor (GR), therefore, resulted in long-term threshold of pain and cognitive improvement.
CONCLUSIONS: Exposure to neonatal repeated procedural pain causes persistent mechanical hypersensitivity and the dysfunction of spatial memory retention at puberty. In addition, glucose can relieve these adverse effects, possibly via decreasing CRF/GR levels to change the hypothalamus-pituitary-adrenal (HPA) axis.
METHODS: Neonatal rats to perform a repetitive injury model and glucose intervention model in the postnatal day 0-7(P0-7). Pain thresholds were measured by von Frey test weekly. The puberty behavioral outcome, tissue loss and protein expression in hippocampus were analyzed.
RESULTS: Oral administration of glucose after repeated pain stimulation can maintain the hippocampal structure in, and reduce the expressions of corticotropin releasing factor (CFR) and glucocorticoid receptor (GR), therefore, resulted in long-term threshold of pain and cognitive improvement.
CONCLUSIONS: Exposure to neonatal repeated procedural pain causes persistent mechanical hypersensitivity and the dysfunction of spatial memory retention at puberty. In addition, glucose can relieve these adverse effects, possibly via decreasing CRF/GR levels to change the hypothalamus-pituitary-adrenal (HPA) axis.
摘要：
背景：新生儿在整个住院期间经历了许多痛苦的手术。反复的手术疼痛可能会导致不良的长期影响。葡萄糖作为一种非药物镇痛，用于新生儿疼痛管理。在这项研究中,研究了减轻由葡萄糖引起的疼痛在新生儿疼痛刺激的神经发育作用中的潜在机制。
方法：新生大鼠在出生后第0-7天（P0-7）进行重复损伤模型和葡萄糖干预模型。每周通过vonFrey测试测量疼痛阈值。青春期的行为结果，分析海马组织丢失和蛋白表达。
结果：反复疼痛刺激后口服葡萄糖可维持海马结构，减少促肾上腺皮质激素释放因子（CFR）和糖皮质激素受体（GR）的表达，因此,导致长期疼痛阈值和认知改善。
结论：暴露于新生儿反复手术疼痛会导致持续的机械性超敏反应和青春期空间记忆保留功能障碍。此外,葡萄糖可以缓解这些副作用，可能通过降低CRF/GR水平来改变下丘脑-垂体-肾上腺（HPA）轴。
方法：新生大鼠在出生后第0-7天（P0-7）进行重复损伤模型和葡萄糖干预模型。每周通过vonFrey测试测量疼痛阈值。青春期的行为结果，分析海马组织丢失和蛋白表达。
结果：反复疼痛刺激后口服葡萄糖可维持海马结构，减少促肾上腺皮质激素释放因子（CFR）和糖皮质激素受体（GR）的表达，因此,导致长期疼痛阈值和认知改善。
结论：暴露于新生儿反复手术疼痛会导致持续的机械性超敏反应和青春期空间记忆保留功能障碍。此外,葡萄糖可以缓解这些副作用，可能通过降低CRF/GR水平来改变下丘脑-垂体-肾上腺（HPA）轴。
