Abstract：
African swine fever virus (ASFV), which was first identified in northern China in 2018, causes high mortality in pigs. Since the I73R protein in ASFV is abundantly expressed during the early phase of virus replication, it can be used as a target protein for early diagnosis. In this study, the I73R protein of ASFV was expressed, and we successfully prepared a novel monoclonal antibody (mAb), 8G11D7, that recognizes this protein. Through both indirect immunofluorescence and Western blotting assays, we demonstrated that 8G11D7 can detect ASFV strains. By evaluating the binding of the antibody to a series of I73R-truncated peptides, the definitive epitope recognized by the monoclonal antibody 8G11D7 was determined to be 58 DKTNTIYPP 66. Bioinformatic analysis revealed that the antigenic epitope had a high antigenic index and conservatism. This study contributes to a deeper understanding of ASFV protein structure and function, helping establish ASFV-specific detection method.
摘要：
非洲猪瘟病毒(ASFV)2018年在中国北方首次发现，导致猪的高死亡率。由于ASFV中的I73R蛋白在病毒复制的早期阶段大量表达，可作为早期诊断的靶蛋白。在这项研究中,ASFV的I73R蛋白表达，我们成功地制备了一种新型的单克隆抗体(mAb),8G11D7，识别该蛋白。通过间接免疫荧光法和蛋白质印迹法,我们证明8G11D7可以检测ASFV株。通过评估抗体与一系列I73R截短的肽的结合，单克隆抗体8G11D7识别的确定表位为58DKTNTIYPP66。生物信息学分析表明，该抗原表位具有较高的抗原指数和保守性。本研究有助于更深入地了解ASFV蛋白的结构和功能,帮助建立ASFV特异性检测方法。
