Abstract：
We examine how oxygen levels and the choice of 16S ribosomal RNA (rRNA) tags impact marine bacterial communities using Next-Generation amplicon sequencing. Analyzing V3 and V6 regions, we assess microbial composition in both Oxygen minimum zones (OMZ) and non-OMZ (NOMZ) areas in the Arabian Sea (AS) and the Central Indian Ocean basin (CIOB) respectively. Operational taxonomic units (OTUs) at 97% similarity showed slightly higher richness and diversity with V6 compared to V3. Vertical diversity patterns were consistent across both regions. NOMZ showed greater richness and diversity than OMZ. AS and CIOB exhibited significant differences in bacterial community, diversity, and relative abundance at the order and family levels. Alteromonadaceae dominated the OMZ, while Pelagibacteraceae dominated the NOMZ. Synechococcaceae were found exclusively at 250 m in OMZ. Bacteria putatively involved in nitrification, denitrification, and sulfurylation were detected at both sites. Dissolved oxygen significantly influenced microbial diversity at both sites, while seasonal environmental parameters affected diversity consistently, with no observed temporal variation.
摘要：
我们使用下一代扩增子测序检查氧水平和16S核糖体RNA（rRNA）标签的选择如何影响海洋细菌群落。分析V3和V6区域，我们分别评估了阿拉伯海（AS）和中印度洋盆地（CIOB）的最小氧气区（OMZ）和非OMZ（NOMZ）区域的微生物组成。与V3相比，V6的操作分类单位（OTU）具有97％的相似性，其丰富度和多样性略高。两个地区的垂直多样性模式是一致的。NOMZ比OMZ显示出更大的丰富度和多样性。AS和CIOB在细菌群落中表现出显著差异，多样性,以及秩序和家庭层面的相对丰富。Alteromonadaceae在OMZ中占主导地位，而鞭毛杆菌科主导了NOMZ。在OMZ仅在250m处发现了Synechococaceae。细菌推测参与硝化作用，反硝化,在两个位点都检测到硫化。溶解氧显著影响两个地点的微生物多样性，虽然季节性环境参数持续影响多样性，没有观察到的时间变化。
