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Abstract：
Photoacoustic computed tomography (PACT), an emerging imaging modality in preclinical cancer research, can provide multiparametric 3D information about structures, physiological functions, and pharmacokinetics. Here, we demonstrate the use of high-definition 3D multiparametric PACT imaging of both primary and metastatic tumors in living mice to noninvasively monitor angiogenesis, carcinogenesis, hypoxia, and pharmacokinetics. The high-definition PACT system with a 1024-element hemispherical ultrasound transducer array provides an isotropic spatial resolution of 380 μm, an effective volumetric field-of-view of 12.8 mm × 12.8 mm × 12.8 mm without scanning, and an acquisition time of <30 s for a whole mouse body. Initially, we monitor the structural progression of the tumor microenvironment (e.g., angiogenesis and vessel tortuosity) after tumor cell inoculation. Then, we analyze the change in oxygen saturation of the tumor during carcinogenesis, verifying induced hypoxia in the tumor\'s core region. Finally, the whole-body pharmacokinetics are photoacoustically imaged after intravenous injection of micelle-loaded IR780 dye, and the in vivo PACT results are validated in vivo and ex vivo by fluorescence imaging. By employing the premium PACT system and applying multiparametric analyses to subcutaneous primary tumors and metastatic liver tumors, we demonstrate that this PACT system can provide multiparametric analyses for comprehensive cancer research.
摘要：
光声计算机断层扫描（PACT），临床前癌症研究中一种新兴的成像模式，可以提供有关结构的多参数3D信息，生理功能，和药代动力学。这里,我们证明了在活体小鼠中使用高清晰度3D多参数PACT成像对原发性和转移性肿瘤进行无创监测血管生成，致癌作用，缺氧,和药代动力学。具有1024个元件的半球形超声换能器阵列的高清PACT系统提供了380μm的各向同性空间分辨率，无扫描的有效体积视场为12.8mm×12.8mm×12.8mm，并且对于整个小鼠身体的采集时间<30s。最初，我们监测肿瘤微环境的结构进展(例如，血管生成和血管弯曲）在肿瘤细胞接种后。然后，我们分析肿瘤癌变过程中氧饱和度的变化，验证肿瘤核心区域的诱导缺氧。最后,在静脉注射胶束负载的IR780染料后，对全身药代动力学进行光声成像，并通过荧光成像在体内和离体验证体内PACT结果。通过采用高级PACT系统并将多参数分析应用于皮下原发性肿瘤和转移性肝肿瘤，我们证明该PACT系统可以为全面的癌症研究提供多参数分析.
