Abstract：
To study how indigenous or probiotic-introduced lactobacilli affect the sensitivity (estimated as the proportion of surviving, apoptotic, and nonapoptotic deaths) of vaginal epithelial cells obtained from HPV-negative and HPV-positive patients to oxidative damage. The tendency to resist oxidative damage in vaginal epithelial cells of 147 HPV-positive and 59 HPV-negative patients with physiological or suboptimal levels of Lactobacillus was evaluated. Adaptation of cell to curb the oxidative damage in 146 HPV positive and 41 HPV negative with probiotic (Lacticaseibacillus rhamnosus Lcr35) supplementation and without was studied. Resistance of epithelial cells to damage was measured by the ratio of surviving, apoptotic, and dead nonapoptotic cells after three times of hydrogen peroxide treatment using a kit containing annexin V-fluorescein in combination with propidium iodide. If uninfected epithelial cells were in an environment with a physiological level of lactobacilli for significant duration, then these cells were more resilient to damage, and if they lost their viability, it was mainly due to apoptosis. Probiotic therapy also increased the resistance of uninfected epithelial cells to damage. HPV-infected epithelial cells were less resistant to damage at normal levels of lactobacilli compared with Lactobacillus deficiency. In HPV-positive patients with Lactobacillus deficiency, probiotic therapy decreased the resistance of infected epithelial cells to damage; the increase in cell death was mainly due to apoptosis.
摘要：
为了研究本地或益生菌引入的乳酸杆菌如何影响敏感性（估计为存活的比例，凋亡，和非凋亡性死亡）从HPV阴性和HPV阳性患者获得的阴道上皮细胞氧化损伤。评估了147名HPV阳性和59名HPV阴性患者的阴道上皮细胞抵抗氧化损伤的趋势，这些患者具有生理或未达到最佳水平的乳杆菌。研究了在补充益生菌（鼠李糖乳杆菌Lcr35）和不补充益生菌的情况下，细胞适应抑制146个HPV阳性和41个HPV阴性的氧化损伤。上皮细胞对损伤的抵抗力是通过存活的比率来衡量的，凋亡，和使用含有膜联蛋白V-荧光素与碘化丙啶的试剂盒进行三次过氧化氢处理后死亡的非凋亡细胞。如果未感染的上皮细胞处于具有生理水平的乳酸杆菌的环境中，然后这些细胞对损伤更有弹性，如果他们失去了生存能力，这主要是由于细胞凋亡。益生菌疗法还增加了未感染的上皮细胞对损伤的抵抗力。与乳杆菌缺乏相比，HPV感染的上皮细胞对正常水平的乳杆菌损伤的抵抗力较低。在乳杆菌缺乏的HPV阳性患者中，益生菌治疗降低了感染的上皮细胞对损伤的抵抗力；细胞死亡的增加主要是由于细胞凋亡。
