PDF(Pubmed)
Abstract：
Niche selection and microbial dispersal are key factors that shape microbial communities. However, their relative significance varies across different environments and spatiotemporal scales. While most studies focus on the impact of these forces on community composition, few consider other structural levels such as the physiological stage of the microbial community and single-cell characteristics. To understand the relative influence of microbial dispersal and niche selection on various community structural levels, we concurrently examined the taxonomic composition, abundance and single-cell characteristics of bacterioplankton in an acidic reservoir (El Sancho, Spain) during stratification and mixing periods. A cluster analysis based on environmental variables identified five niches during stratification and one during mixing. Canonical correspondence analysis (CCA) revealed that communities within each niche differed in both, taxonomic and single-cell characteristics. The environmental variables that explained the variation in class-based ordination differed from those explaining the ordination based on single-cell characteristics. However, a Procrustes analysis indicated a high correlation between the CCA ordinations based on both structural levels, suggesting simultaneous changes in the microbial community at multiple structural levels. Our findings underscore the dominant role of environmental selection in occupying different microbial niches, given that microbial dispersal was not restricted.
摘要：
生态位选择和微生物分散是形成微生物群落的关键因素。然而,它们的相对意义在不同的环境和时空尺度上有所不同。虽然大多数研究集中在这些力量对社区组成的影响，很少考虑其他结构水平，例如微生物群落的生理阶段和单细胞特征。为了了解微生物分散和生态位选择对各种群落结构水平的相对影响，我们同时检查了分类组成，酸性储层中浮游细菌的丰度和单细胞特征(ElSancho，西班牙)在分层和混合期间。基于环境变量的聚类分析在分层过程中确定了五个生态位，在混合过程中确定了一个。规范对应分析(CCA)表明，每个生态位内的社区在两个方面都不同，分类学和单细胞特征。解释基于类别的排序变化的环境变量与解释基于单细胞特征的排序的环境变量不同。然而,Procrustes分析表明，基于两个结构水平的CCA坐标之间存在高度相关性，表明微生物群落在多个结构水平上同时变化。我们的发现强调了环境选择在占据不同微生物生态位方面的主导作用，鉴于微生物扩散不受限制。
