PDF(Pubmed)
Abstract：
Extracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles and low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration of human cell-derived EVs into pig-tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation time, and may thus hamper successful EV-mediated cargo delivery into tissues. Here, we share the custom ELISA protocol that we used to measure such antibody responses. This protocol may help other researchers evaluate immune responses to EV-based therapies in preclinical studies.
摘要：
对细胞外囊泡(EV)的治疗潜力和作为药物递送载体的应用进行了深入研究。良好的安全性和低免疫原性的广泛认知使电动汽车成为合成纳米颗粒的有吸引力的替代品。我们最近表明，在三次或更多次注射后，将人细胞衍生的EV反复静脉内注射到猪尾猕猴中，意外地引发了抗体反应。这与EV循环时间的减少相吻合，并因此可能妨碍成功的EV介导的货物递送到组织中。这里,我们共享用于测量此类抗体应答的自定义ELISA方案.该方案可以帮助其他研究人员在临床前研究中评估对基于EV的疗法的免疫反应。
