PDF(Pubmed)
Abstract：
UNASSIGNED: Urinary extracellular vesicles (uEVs) can be considered biomarkers of kidney diseases. EVs derived from podocytes may reflect podocyte damage in different glomerular diseases. IgA nephropathy (IgAN) is one of the most common forms of glomerulonephritis (GN) characterized by proteinuria and hematuria. This study aimed to analyze the uEVs of IgAN patients to understand the pathophysiological processes of the disease at the protein level.
UNASSIGNED: Patients with GN [biopsy-proven IgAN (n = 16) and membranous glomerulonephritis (MGN, n = 16)], and healthy controls (n = 16) were included in this study. The uEVs were extracted, characterized, and analyzed to evaluate the protein levels of candidate markers of IgAN, including vasorin precursor, aminopeptidase N, and ceruloplasmin by western-blot analysis.
UNASSIGNED: Higher levels of both podocytes and EVs-related proteins were observed in the pooled urine samples of GN patients compared to the healthy controls. In IgAN patients, uEV-protein levels of vasorin were statistically lower while levels of ceruloplasmin were significantly higher compared to MGN (P = 0.002, P = 0.06) and healthy controls, respectively (P = 0.020, P= 0.001).
UNASSIGNED: Different levels of the studied proteins in uEVs may indicate podocyte injury and represent a direct association with the pathology of IgAN and MGN.
UNASSIGNED: Urinary extracellular vesicles (uEVs) can be considered biomarkers of kidney diseases. EVs derived from podocytes may reflect podocyte damage in different glomerular diseases. IgA nephropathy (IgAN) is one of the most common forms of glomerulonephritis (GN) characterized by proteinuria and hematuria. This study aimed to analyze the uEVs of IgAN patients to understand the pathophysiological processes of the disease at the protein level.
UNASSIGNED: Patients with GN [biopsy-proven IgAN (n = 16) and membranous glomerulonephritis (MGN, n = 16)], and healthy controls (n = 16) were included in this study. The uEVs were extracted, characterized, and analyzed to evaluate the protein levels of candidate markers of IgAN, including vasorin precursor, aminopeptidase N, and ceruloplasmin by western-blot analysis.
UNASSIGNED: Higher levels of both podocytes and EVs-related proteins were observed in the pooled urine samples of GN patients compared to the healthy controls. In IgAN patients, uEV-protein levels of vasorin were statistically lower while levels of ceruloplasmin were significantly higher compared to MGN (P = 0.002, P = 0.06) and healthy controls, respectively (P = 0.020, P= 0.001).
UNASSIGNED: Different levels of the studied proteins in uEVs may indicate podocyte injury and represent a direct association with the pathology of IgAN and MGN.
摘要：
■尿液细胞外囊泡（uEV）可以被认为是肾脏疾病的生物标志物。来自足细胞的EV可能反映了不同肾小球疾病中足细胞的损害。IgA肾病（IgAN）是以蛋白尿和血尿为特征的肾小球肾炎（GN）的最常见形式之一。本研究旨在分析IgAN患者的uEV，以了解该疾病在蛋白质水平上的病理生理过程。
■患有GN[活检证实的IgAN（n=16）和膜性肾小球肾炎（MGN，n=16)],和健康对照(n=16)纳入本研究。提取了uEV，characterized,并分析以评估IgAN候选标志物的蛋白质水平，包括血管蛋白前体,氨基肽酶N,和铜蓝蛋白通过蛋白质印迹分析。
■与健康对照相比，在GN患者的合并尿液样本中观察到更高水平的足细胞和EV相关蛋白。在IgAN患者中,与MGN（P=0.002，P=0.06）和健康对照组相比，血管蛋白的uEV蛋白水平在统计学上较低，而铜蓝蛋白水平则显着较高，(P=0.020,P=0.001)。
■uEV中研究的蛋白质的不同水平可能表明足细胞损伤，并表示与IgAN和MGN的病理直接相关。
■尿液细胞外囊泡（uEV）可以被认为是肾脏疾病的生物标志物。来自足细胞的EV可能反映了不同肾小球疾病中足细胞的损害。IgA肾病（IgAN）是以蛋白尿和血尿为特征的肾小球肾炎（GN）的最常见形式之一。本研究旨在分析IgAN患者的uEV，以了解该疾病在蛋白质水平上的病理生理过程。
■患有GN[活检证实的IgAN（n=16）和膜性肾小球肾炎（MGN，n=16)],和健康对照(n=16)纳入本研究。提取了uEV，characterized,并分析以评估IgAN候选标志物的蛋白质水平，包括血管蛋白前体,氨基肽酶N,和铜蓝蛋白通过蛋白质印迹分析。
■与健康对照相比，在GN患者的合并尿液样本中观察到更高水平的足细胞和EV相关蛋白。在IgAN患者中,与MGN（P=0.002，P=0.06）和健康对照组相比，血管蛋白的uEV蛋白水平在统计学上较低，而铜蓝蛋白水平则显着较高，(P=0.020,P=0.001)。
■uEV中研究的蛋白质的不同水平可能表明足细胞损伤，并表示与IgAN和MGN的病理直接相关。
