PDF(Pubmed)
Abstract：
UNASSIGNED: The intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors.
UNASSIGNED: Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with the phospholipase C zeta (PLCɀ) activity of both species.
UNASSIGNED: In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples.
UNASSIGNED: Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages and ICSI + chemical activation presented the highest 2 PN, cleavage, and blastocyst rates (p < 0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI (p < 0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm (p < 0.05). Higher values of PLCɀ activity were found in human sperm compared with bovine sperm (p < 0.05).
UNASSIGNED: Our results suggest that the higher stability of the bovine sperm in combination with its relatively low content of PLCɀ impairs bovine oocyte activation after ICSI.
UNASSIGNED: Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with the phospholipase C zeta (PLCɀ) activity of both species.
UNASSIGNED: In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples.
UNASSIGNED: Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages and ICSI + chemical activation presented the highest 2 PN, cleavage, and blastocyst rates (p < 0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI (p < 0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm (p < 0.05). Higher values of PLCɀ activity were found in human sperm compared with bovine sperm (p < 0.05).
UNASSIGNED: Our results suggest that the higher stability of the bovine sperm in combination with its relatively low content of PLCɀ impairs bovine oocyte activation after ICSI.
摘要：
■卵胞浆内单精子注射（ICSI）技术在牛中效率低。这主要归因于卵母细胞和/或精子因素导致的卵母细胞活化失败。
■我们的目的是评估常规ICSI和带有公牛或人类精子的Piezo-ICSI对牛卵母细胞活化和胚胎发育的影响，并评估其与磷脂酶Cζ（PLC）的关系。两种物种的活性。
■将体外成熟的牛卵母细胞随机分为五组，并按以下方式受精：常规ICSI使用具有化学活化作用的牛精子（对照），使用牛精子的常规ICSI，使用牛精子的压电ICSI，使用人类精子的常规ICSI，和使用人类精子的Piezo-ICSI。测定了公牛和人精子样品中的PLC活性。
■在使用公牛精子的群体中，通过常规ICSI受精的卵母细胞具有2个原核（PN）形成和卵裂的最低值，Piezo-ICSI增加了两个百分比，ICSI+化学活化呈现最高的2PN，乳沟,和囊胚率（p<0.05）。在使用人类精子的群体中，Piezo-ICSI受精的卵母细胞比常规ICSI激活的卵母细胞具有更高的2PN和卵裂率（p<0.05）。具有人精子的Piezo-ICSI增加牛卵母细胞活化与具有牛精子的常规ICSI+化学活化一样多(p<0.05)。与牛精子相比，人精子中的PLC活性值较高（p<0.05）。
■我们的结果表明，牛精子的较高稳定性与其相对较低的PLC含量相结合会损害ICSI后牛卵母细胞的活化。
■我们的目的是评估常规ICSI和带有公牛或人类精子的Piezo-ICSI对牛卵母细胞活化和胚胎发育的影响，并评估其与磷脂酶Cζ（PLC）的关系。两种物种的活性。
■将体外成熟的牛卵母细胞随机分为五组，并按以下方式受精：常规ICSI使用具有化学活化作用的牛精子（对照），使用牛精子的常规ICSI，使用牛精子的压电ICSI，使用人类精子的常规ICSI，和使用人类精子的Piezo-ICSI。测定了公牛和人精子样品中的PLC活性。
■在使用公牛精子的群体中，通过常规ICSI受精的卵母细胞具有2个原核（PN）形成和卵裂的最低值，Piezo-ICSI增加了两个百分比，ICSI+化学活化呈现最高的2PN，乳沟,和囊胚率（p<0.05）。在使用人类精子的群体中，Piezo-ICSI受精的卵母细胞比常规ICSI激活的卵母细胞具有更高的2PN和卵裂率（p<0.05）。具有人精子的Piezo-ICSI增加牛卵母细胞活化与具有牛精子的常规ICSI+化学活化一样多(p<0.05)。与牛精子相比，人精子中的PLC活性值较高（p<0.05）。
■我们的结果表明，牛精子的较高稳定性与其相对较低的PLC含量相结合会损害ICSI后牛卵母细胞的活化。
