PDF(Pubmed)
Abstract：
UNASSIGNED: This research describes the methodology used for the preparation of selenium nanoparticles from Pseudomonas aeruginosa and their administration to lambs for lipid profile checking, administration of selenium nanoparticles as a medication in lambs results in hypolipidemia.
UNASSIGNED: The study aimed to investigate the potential of selenium nanoparticles in improving lipid profiles in lambs.
UNASSIGNED: Healthy lambs (n = 10) of similar age and weight were selected for the study. The animals were housed in individual pens with free access to water and a standard diet. The lambs were randomly divided into two groups: the control group (n = 5) and the treatment group (n = 5). The control group received a standard diet, while the treatment group received the same diet and oral administrated with selenium nanoparticles at 0.1 mg/kg body weight. The administration was carried out daily for a period of 8 weeks. Blood samples were collected from the jugular vein of each lamb at the beginning of the study (baseline) and at the end of the 2 weeks treatment period. The samples were collected in vacutainer tubes and allowed to clot. Serum was separated by centrifugation at 3,000 rpm for 10 minutes and stored at -80°C for estimation of lipid profile total cholesterol (TC), triglyceride, high-density lipoprotein (HDL), and low-density lipoprotein (LDL). The serum samples were used for the estimation of lipid profile levels using an enzymatic colorimetric method. The absorbance was measured at 540 nm using a spectrophotometer.
UNASSIGNED: The results showed a significant decrease in serum TC, triglyceride, and very-low-density lipoprotein cholesterol levels after selenium nanoparticle supplementation compared to the control group (p < 0.05), the results indicated a significant increase in serum HDL levels after selenium nanoparticle supplementation compared to the control group (p < 0.05). This indicates that selenium nanoparticle supplementation has a beneficial effect on reducing TC levels in lambs.
UNASSIGNED: The conclusion section will summarize the findings of the study and highlight the potential of selenium nanoparticles in improving lipid profiles in lambs. The implications of the study for animal nutrition and health will be discussed, along with the need for further research in this area.
UNASSIGNED: The study aimed to investigate the potential of selenium nanoparticles in improving lipid profiles in lambs.
UNASSIGNED: Healthy lambs (n = 10) of similar age and weight were selected for the study. The animals were housed in individual pens with free access to water and a standard diet. The lambs were randomly divided into two groups: the control group (n = 5) and the treatment group (n = 5). The control group received a standard diet, while the treatment group received the same diet and oral administrated with selenium nanoparticles at 0.1 mg/kg body weight. The administration was carried out daily for a period of 8 weeks. Blood samples were collected from the jugular vein of each lamb at the beginning of the study (baseline) and at the end of the 2 weeks treatment period. The samples were collected in vacutainer tubes and allowed to clot. Serum was separated by centrifugation at 3,000 rpm for 10 minutes and stored at -80°C for estimation of lipid profile total cholesterol (TC), triglyceride, high-density lipoprotein (HDL), and low-density lipoprotein (LDL). The serum samples were used for the estimation of lipid profile levels using an enzymatic colorimetric method. The absorbance was measured at 540 nm using a spectrophotometer.
UNASSIGNED: The results showed a significant decrease in serum TC, triglyceride, and very-low-density lipoprotein cholesterol levels after selenium nanoparticle supplementation compared to the control group (p < 0.05), the results indicated a significant increase in serum HDL levels after selenium nanoparticle supplementation compared to the control group (p < 0.05). This indicates that selenium nanoparticle supplementation has a beneficial effect on reducing TC levels in lambs.
UNASSIGNED: The conclusion section will summarize the findings of the study and highlight the potential of selenium nanoparticles in improving lipid profiles in lambs. The implications of the study for animal nutrition and health will be discussed, along with the need for further research in this area.
摘要：
■这项研究描述了用于从铜绿假单胞菌中制备硒纳米颗粒的方法，以及将其给予羔羊进行脂质分布检查的方法，在羔羊中使用硒纳米颗粒作为药物会导致低脂血症。
■该研究旨在研究硒纳米颗粒在改善羔羊脂质分布中的潜力。
■选择年龄和体重相似的健康羔羊(n=10)进行研究。将动物饲养在单独的围栏中,自由接触水和标准饮食。将羔羊随机分为对照组(n=5)和治疗组(n=5)。对照组接受标准饮食，而治疗组接受相同的饮食和口服0.1mg/kg体重的硒纳米颗粒。每天进行给药,持续8周。在研究开始时(基线)和2周治疗期结束时,从每只羔羊的颈静脉收集血样。将样品收集在vacutainer管中并使其凝结。通过在3,000rpm下离心分离血清10分钟，并在-80°C下储存以评估脂质概况总胆固醇（TC）。甘油三酯，高密度脂蛋白（HDL），低密度脂蛋白（LDL）。血清样品用于使用酶比色法估计脂质分布水平。使用分光光度计在540nm处测量吸光度。
■结果显示血清TC显著下降，甘油三酯，补硒后极低密度脂蛋白胆固醇水平与对照组相比(p<0.05),结果表明，与对照组相比，纳米硒补充后血清HDL水平显着增加（p<0.05）。这表明硒纳米颗粒补充对降低羔羊的TC水平具有有益作用。
■结论部分将总结研究结果，并强调硒纳米颗粒在改善羔羊脂质分布方面的潜力。将讨论这项研究对动物营养和健康的影响，随着这方面进一步研究的需要。
■该研究旨在研究硒纳米颗粒在改善羔羊脂质分布中的潜力。
■选择年龄和体重相似的健康羔羊(n=10)进行研究。将动物饲养在单独的围栏中,自由接触水和标准饮食。将羔羊随机分为对照组(n=5)和治疗组(n=5)。对照组接受标准饮食，而治疗组接受相同的饮食和口服0.1mg/kg体重的硒纳米颗粒。每天进行给药,持续8周。在研究开始时(基线)和2周治疗期结束时,从每只羔羊的颈静脉收集血样。将样品收集在vacutainer管中并使其凝结。通过在3,000rpm下离心分离血清10分钟，并在-80°C下储存以评估脂质概况总胆固醇（TC）。甘油三酯，高密度脂蛋白（HDL），低密度脂蛋白（LDL）。血清样品用于使用酶比色法估计脂质分布水平。使用分光光度计在540nm处测量吸光度。
■结果显示血清TC显著下降，甘油三酯，补硒后极低密度脂蛋白胆固醇水平与对照组相比(p<0.05),结果表明，与对照组相比，纳米硒补充后血清HDL水平显着增加（p<0.05）。这表明硒纳米颗粒补充对降低羔羊的TC水平具有有益作用。
■结论部分将总结研究结果，并强调硒纳米颗粒在改善羔羊脂质分布方面的潜力。将讨论这项研究对动物营养和健康的影响，随着这方面进一步研究的需要。
