PDF(Pubmed)
Abstract：
UNASSIGNED: Canine Legg Calvé Perthes disease (LCPD) occurs during the growth period, and the cause of ischemic necrosis of the femoral head during growth remains unclear. If LCPD-affected femoral head-derived mesenchymal stem cells (LCPD-MSCs) can be generated, they can be used as a new tool for the pathophysiological analysis of canine LCPD.
UNASSIGNED: To generate affected femoral head-derived mesenchymal stem cells (MSCs) from dogs with LCPD and investigate the mRNA expression levels of angiogenesis-related factors and osteogenic differentiation potency of LCPD-MSCs.
UNASSIGNED: This study was performed using affected femoral heads from dogs diagnosed with LCPD and underwent femoral head and neck ostectomy. The necrotic tissue was harvested from the LCPD-affected femoral head and cultured statically (LCPD group, n = 6). Canine bone marrow-derived MSCs (BM-MSCs) were used as controls (control group, n = 6). First, the morphology of the cultured cells was observed, and the expression of CD29, CD34, CD44, CD45, CD90, and major histocompatibility complex class II was analyzed using flow cytometry. Additionally, the trilineage differentiation potency of the LCPD-affected head-derived adherent cells was examined. Furthermore, the expression levels of HIF1A, VEGFA, VEGFB, and PDGFB mRNAs and the bone differentiation potency of LCPD-affected head-derived adherent cells were investigated.
UNASSIGNED: LCPD-affected femoral head-derived adherent cells showed a fibroblast-like morphology, and the expression of cell surface antigens was similar to that of BM-MSCs. In addition, LCPD-affected femoral head-derived adherent cells showed the same trilineage differentiation potency as BM-MSCs and were consistent with MSC characteristics. Furthermore, the mRNA expression levels of angiogenesis-related factors could be objectively measured in LCPD-MSCs and those MSCs had bone differentiation potency.
UNASSIGNED: In the present study, canine LCPD-MSCs were successfully generated, suggesting their usefulness as a tool for pathological analysis of LCPD in dogs.
UNASSIGNED: To generate affected femoral head-derived mesenchymal stem cells (MSCs) from dogs with LCPD and investigate the mRNA expression levels of angiogenesis-related factors and osteogenic differentiation potency of LCPD-MSCs.
UNASSIGNED: This study was performed using affected femoral heads from dogs diagnosed with LCPD and underwent femoral head and neck ostectomy. The necrotic tissue was harvested from the LCPD-affected femoral head and cultured statically (LCPD group, n = 6). Canine bone marrow-derived MSCs (BM-MSCs) were used as controls (control group, n = 6). First, the morphology of the cultured cells was observed, and the expression of CD29, CD34, CD44, CD45, CD90, and major histocompatibility complex class II was analyzed using flow cytometry. Additionally, the trilineage differentiation potency of the LCPD-affected head-derived adherent cells was examined. Furthermore, the expression levels of HIF1A, VEGFA, VEGFB, and PDGFB mRNAs and the bone differentiation potency of LCPD-affected head-derived adherent cells were investigated.
UNASSIGNED: LCPD-affected femoral head-derived adherent cells showed a fibroblast-like morphology, and the expression of cell surface antigens was similar to that of BM-MSCs. In addition, LCPD-affected femoral head-derived adherent cells showed the same trilineage differentiation potency as BM-MSCs and were consistent with MSC characteristics. Furthermore, the mRNA expression levels of angiogenesis-related factors could be objectively measured in LCPD-MSCs and those MSCs had bone differentiation potency.
UNASSIGNED: In the present study, canine LCPD-MSCs were successfully generated, suggesting their usefulness as a tool for pathological analysis of LCPD in dogs.
摘要：
■犬LeggCalvéPerthes病（LCPD）发生在生长期，股骨头生长过程中缺血性坏死的原因尚不清楚。如果可以产生受LCPD影响的股骨头间充质干细胞（LCPD-MSCs），它们可以作为犬LCPD病理生理分析的新工具。
■从LCPD犬产生受影响的股骨头间充质干细胞（MSCs），并研究血管生成相关因子的mRNA表达水平和LCPD-MSCs的成骨分化潜能。
■这项研究是使用诊断为LCPD的狗的受影响的股骨头进行的，并接受了股骨头和颈骨切除术。从受LCPD影响的股骨头收集坏死组织并静态培养（LCPD组，n=6)。犬骨髓间充质干细胞(BM-MSCs)作为对照(对照组,n=6)。首先,观察培养细胞的形态,使用流式细胞术分析CD29，CD34，CD44，CD45，CD90和主要组织相容性复合物II类的表达。此外,检查了受LCPD影响的头部来源的贴壁细胞的三系分化能力。此外，HIF1A的表达水平，VEGFA,VEGFB,研究了受LCPD影响的头部来源的贴壁细胞的PDGFBmRNA和骨分化潜能。
■受LCPD影响的股骨头来源的贴壁细胞显示成纤维细胞样形态，细胞表面抗原的表达与BM-MSCs相似。此外,受LCPD影响的股骨头来源的贴壁细胞显示出与BM-MSC相同的三系分化能力，并且与MSC特征一致。此外，血管生成相关因子的mRNA表达水平可以在LCPD-MSCs中进行客观测量,这些MSCs具有骨分化潜能.
■在本研究中，成功生成犬LCPD-MSCs,表明它们作为狗LCPD病理分析工具的有用性。
■从LCPD犬产生受影响的股骨头间充质干细胞（MSCs），并研究血管生成相关因子的mRNA表达水平和LCPD-MSCs的成骨分化潜能。
■这项研究是使用诊断为LCPD的狗的受影响的股骨头进行的，并接受了股骨头和颈骨切除术。从受LCPD影响的股骨头收集坏死组织并静态培养（LCPD组，n=6)。犬骨髓间充质干细胞(BM-MSCs)作为对照(对照组,n=6)。首先,观察培养细胞的形态,使用流式细胞术分析CD29，CD34，CD44，CD45，CD90和主要组织相容性复合物II类的表达。此外,检查了受LCPD影响的头部来源的贴壁细胞的三系分化能力。此外，HIF1A的表达水平，VEGFA,VEGFB,研究了受LCPD影响的头部来源的贴壁细胞的PDGFBmRNA和骨分化潜能。
■受LCPD影响的股骨头来源的贴壁细胞显示成纤维细胞样形态，细胞表面抗原的表达与BM-MSCs相似。此外,受LCPD影响的股骨头来源的贴壁细胞显示出与BM-MSC相同的三系分化能力，并且与MSC特征一致。此外，血管生成相关因子的mRNA表达水平可以在LCPD-MSCs中进行客观测量,这些MSCs具有骨分化潜能.
■在本研究中，成功生成犬LCPD-MSCs,表明它们作为狗LCPD病理分析工具的有用性。
