Abstract：
Insecticide susceptibility is mainly determined by the insect host, but symbiotic bacteria are also an important affecting factor. In this study, we investigate the relationship between the structure of gut bacterial symbionts and insecticide susceptibility in Diaphorina citri, the important carrier of Candidatus Liberibacter asiaticus (CLas), the causal agent of Huanglongbing (HLB). Our results indicated that antibiotic treatment significantly increased the susceptibility of D. citri to bifenthrin and thiamethoxam, and significantly decreased the relative abundance of Wolbachia and Profftella, enzyme activities of CarEs, and expression level of multiple CarE genes. The relative loads of Wolbachia and Profftella were positively correlated with DcitCCE13, DcitCCE14, DcitCCE15, and DcitCCE16. RNAi and prokaryotic expression revealed that DcitCCE15 is associated with bifenthrin metabolism. These results revealed that bacterial symbionts might regulate DcitCCE15 expression, which is involved in the susceptibility of D. citri to bifenthrin.
摘要：
杀虫剂的敏感性主要由昆虫宿主决定，但是共生细菌也是一个重要的影响因素。在这项研究中,我们研究了柑橘中肠道细菌共生体的结构与杀虫剂敏感性之间的关系，亚洲念珠菌（CLAS）的重要载体，黄龙病的病原体(HLB)。我们的结果表明，抗生素治疗显着增加了D.citri对联苯菊酯和噻虫嗪的敏感性，并且显著降低了Wolbachia和Profftella的相对丰度，CarEs的酶活性，和多个CarE基因的表达水平。Wolbachia和Profftella的相对负荷与DcitCCE13，DcitCCE14，DcitCCE15和DcitCCE16呈正相关。RNAi和原核表达表明DcitCCE15与联苯菊酯的代谢有关。这些结果表明，细菌共生体可能调节DcitCCE15的表达，这与D.citri对联苯菊酯的敏感性有关。
