Abstract：
Ovarian theca cells produce testosterone, which acts as a vital precursor substance for synthesizing estrogens during follicular development. Nerve growth factor (NGF) has been shown to participate in reproductive physiology, specifically to follicular development and ovulation. There is currently no available data on the impact of NGF on testosterone synthesis in porcine theca cells. Furthermore, m6A modification is the most common internal modification in eukaryotic mRNAs that are closely associated with female gametogenesis, follicle development, ovulation, and other related processes. It is also uncertain whether the three main enzymes associated with m6A, such as Writers, Erasers and Readers, play a role in this process. The present study, with an in vitro culture model, investigated the effect of NGF on testosterone synthesis in porcine theca cells and the role of Writers-METTL14 in this process. It was found that NGF activates the PI3K/AKT signaling pathway through METTL14, which regulates testosterone synthesis in porcine theca cells. This study will help to further elucidate the mechanisms by which NGF regulates follicular development and provide new therapeutic targets for ovary-related diseases in female animals.
摘要：
卵巢卵泡膜细胞产生睾酮，作为卵泡发育过程中合成雌激素的重要前体物质。神经生长因子（NGF）已被证明参与生殖生理，特别是卵泡发育和排卵。目前没有关于NGF对猪卵泡膜细胞中睾酮合成的影响的可用数据。此外，m6A修饰是与雌配子发生密切相关的真核mRNAs中最常见的内部修饰，卵泡发育,排卵,和其他相关过程。还不确定与m6A相关的三种主要酶，比如作家,橡皮擦和读者,在这个过程中发挥作用。本研究,用体外培养模型，研究了NGF对猪卵泡膜细胞睾酮合成的影响以及Writers-METTL14在此过程中的作用。发现NGF通过METTL14激活PI3K/AKT信号通路,其调节猪卵泡膜细胞中的睾酮合成。本研究将有助于进一步阐明NGF调控卵泡发育的机制，为雌性动物卵巢相关疾病提供新的治疗靶点。
