Abstract：
UNASSIGNED: The objective was to investigate growth performance, antioxidant enzyme activity, intestinal morphology, immune cell distribution, short chain fatty acid (SCFA) profile, and microbiota in broiler chickens fed a diet containing Lacticaseibacillus paracasei NSMJ15.
UNASSIGNED: A total of 120-day-old Ross 308 male broilers were allocated to 2 dietary treatments in a randomized complete block design. A control group was fed a corn-soybean meal control diet, and an NSMJ15-supplemented group was fed a control diet supplemented with 1 g/kg L. paracasei NSMJ15 at the expense of cornstarch. Each dietary treatment had 6 replicates with 10 birds per cage. Growth performance was recorded on day 9. On day 10, one bird representing median body weight was selected to collect serum for antioxidant enzyme activity, jejunal tissue for immune cell isolation and morphometric analysis, and cecal digesta for 16S rRNA gene sequencing and SCFA analysis.
UNASSIGNED: Supplementation of L. paracasei NSMJ15 did not affect growth performance, serum antioxidant enzyme activity, and jejunal histomorphology compared to the control group. In the NSMJ15-supplemented group, the population of CD3+CD4+CD8- T cells increased (p=0.010), while the population of CD3+CD8+TCRγδ+ T cells decreased (p=0.022) compared to the control group. The L. paracasei NSMJ15 supplementation decreased (p=0.022) acetate concentration in the cecal digesta compared to the control group. The 16S rRNA gene sequencing analysis showed that NSMJ15-supplemented group differentially expressed (p<0.05) 10 more amplicon sequence variants compared to control group without affecting alpha and beta diversity indices of the cecal microbiota. Genera Mediterraneibacter and Negativibacillus were positively (p<0.05) correlated with CD4+ T cells, while genera Gemmiger, Coprococcus, Sellimonas, Massilimicrobiota, and Blautia were negatively (p<0.05) correlated with SCFA concentration.
UNASSIGNED: The results of the present study suggest dietary L. paracasei NSMJ15 supplementation may increase percentage of CD4+ T cells and decrease acetate concentration in broiler chickens by increasing the differential expression of specific microbial genera.
UNASSIGNED: A total of 120-day-old Ross 308 male broilers were allocated to 2 dietary treatments in a randomized complete block design. A control group was fed a corn-soybean meal control diet, and an NSMJ15-supplemented group was fed a control diet supplemented with 1 g/kg L. paracasei NSMJ15 at the expense of cornstarch. Each dietary treatment had 6 replicates with 10 birds per cage. Growth performance was recorded on day 9. On day 10, one bird representing median body weight was selected to collect serum for antioxidant enzyme activity, jejunal tissue for immune cell isolation and morphometric analysis, and cecal digesta for 16S rRNA gene sequencing and SCFA analysis.
UNASSIGNED: Supplementation of L. paracasei NSMJ15 did not affect growth performance, serum antioxidant enzyme activity, and jejunal histomorphology compared to the control group. In the NSMJ15-supplemented group, the population of CD3+CD4+CD8- T cells increased (p=0.010), while the population of CD3+CD8+TCRγδ+ T cells decreased (p=0.022) compared to the control group. The L. paracasei NSMJ15 supplementation decreased (p=0.022) acetate concentration in the cecal digesta compared to the control group. The 16S rRNA gene sequencing analysis showed that NSMJ15-supplemented group differentially expressed (p<0.05) 10 more amplicon sequence variants compared to control group without affecting alpha and beta diversity indices of the cecal microbiota. Genera Mediterraneibacter and Negativibacillus were positively (p<0.05) correlated with CD4+ T cells, while genera Gemmiger, Coprococcus, Sellimonas, Massilimicrobiota, and Blautia were negatively (p<0.05) correlated with SCFA concentration.
UNASSIGNED: The results of the present study suggest dietary L. paracasei NSMJ15 supplementation may increase percentage of CD4+ T cells and decrease acetate concentration in broiler chickens by increasing the differential expression of specific microbial genera.
摘要：
■目的是调查增长绩效，抗氧化酶活性，肠道形态学,免疫细胞分布，短链脂肪酸（SCFA）谱，饲喂含有副干酪乳杆菌NSMJ15的日粮的肉鸡和微生物群。
■在随机完全区组设计中将总共120天大的Ross308雄性肉鸡分配给2种饮食处理。对照组饲喂玉米豆粕对照日粮,补充NSMJ15的组以玉米淀粉为代价，饲喂补充1g/kg副干酪乳杆菌NSMJ15的对照饮食。每种饮食处理具有6个重复,每个笼10只鸟。在第9天记录生长表现。在第10天，选择一只代表中位体重的鸟收集血清进行抗氧化酶活性，免疫细胞分离和形态分析的空肠组织，和盲肠消化进行16SrRNA基因测序和SCFA分析。
■副干酪乳杆菌NSMJ15的补充不影响生长性能，血清抗氧化酶活性，空肠组织形态学与对照组比较。在NSMJ15补充的组中，CD3+CD4+CD8-T细胞的数量增加（p=0.010），与对照组相比，CD3+CD8+TCRγδ+T细胞的数量减少（p=0.022）。与对照组相比，补充副干酪乳杆菌NSMJ15降低了盲肠消化物中的乙酸浓度（p=0.022）。16SrRNA基因测序分析表明，与对照组相比，NSMJ15补充组差异表达（p<0.05）10个扩增子序列变体，而不影响盲肠微生物群的α和β多样性指数。地中海杆菌属和阴杆菌属与CD4+T细胞呈正相关(p<0.05),而Gemmiger属,球菌,塞利莫纳斯,微生物菌群,Blautia与SCFA浓度呈负相关（p<0.05）。
■本研究的结果表明，饲粮中添加副干酪乳杆菌NSMJ15可以通过增加特定微生物属的差异表达来增加肉鸡的CD4T细胞百分比并降低乙酸盐浓度。
■在随机完全区组设计中将总共120天大的Ross308雄性肉鸡分配给2种饮食处理。对照组饲喂玉米豆粕对照日粮,补充NSMJ15的组以玉米淀粉为代价，饲喂补充1g/kg副干酪乳杆菌NSMJ15的对照饮食。每种饮食处理具有6个重复,每个笼10只鸟。在第9天记录生长表现。在第10天，选择一只代表中位体重的鸟收集血清进行抗氧化酶活性，免疫细胞分离和形态分析的空肠组织，和盲肠消化进行16SrRNA基因测序和SCFA分析。
■副干酪乳杆菌NSMJ15的补充不影响生长性能，血清抗氧化酶活性，空肠组织形态学与对照组比较。在NSMJ15补充的组中，CD3+CD4+CD8-T细胞的数量增加（p=0.010），与对照组相比，CD3+CD8+TCRγδ+T细胞的数量减少（p=0.022）。与对照组相比，补充副干酪乳杆菌NSMJ15降低了盲肠消化物中的乙酸浓度（p=0.022）。16SrRNA基因测序分析表明，与对照组相比，NSMJ15补充组差异表达（p<0.05）10个扩增子序列变体，而不影响盲肠微生物群的α和β多样性指数。地中海杆菌属和阴杆菌属与CD4+T细胞呈正相关(p<0.05),而Gemmiger属,球菌,塞利莫纳斯,微生物菌群,Blautia与SCFA浓度呈负相关（p<0.05）。
■本研究的结果表明，饲粮中添加副干酪乳杆菌NSMJ15可以通过增加特定微生物属的差异表达来增加肉鸡的CD4T细胞百分比并降低乙酸盐浓度。
