Abstract：
UNASSIGNED: This study aimed to determine the effects of compatibility of Clostridium butyricum and Bacillus subtilis on growth performance, lipid metabolism, antioxidant status and cecal microflora of broilers during the starter phase.
UNASSIGNED: A total of 600 1-day-old Ross 308 broilers were randomly divided into two groups with six replicates in each group. Chickens in the control group were fed a basal diet, while chickens in the experimental group were fed a diet supplemented with 2 × 108 CFU/kg of C. butyricum and 1 × 109 CFU/kg of B. subtilis. The experimental period was 21 days.
UNASSIGNED: Addition of C. butyricum and B. subtilis significantly increased (p<0.05) the body weight and liver NADP-malic enzyme (NADP-ME) activity of broilers, enhanced (p<0.05) the average daily gain and average daily feed intake of broilers. However, the addition of C. butyricum and B. subtilis did not significantly affect the concentrations of triglyceride and total cholesterol in the serum, the activities of fatty acid synthase and acetyl-CoA carboxylase in the liver, the total antioxidant capacity, glutathione peroxidase activity and malondialdehyde content in the serum and liver. Besides, microbial analysis revealed that supplementation of C. butyricum and B. subtilis increased (p<0.05) the abundance of Firmicutes such as CHKCI001 and Faecalibacterium, decreased (p<0.05) the abundance of Bacteroidota such as Bacteroides and Alistipes. Spearman correlation analysis confirmed that the above cecal microbiota were closely related to the growth performance of broilers (p<0.05). In addition, simultaneous supplementation of C. butyricum and B. subtilis significant affected (p<0.05) 33 different functional pathways such as lipid metabolism and carbohydrate metabolism. This explains the phenomenon of increased growth performance and liver NADP-ME activity in the probiotics group.
UNASSIGNED: The compatibility of C. butyricum and B. subtilis could improve the growth of broilers during the starter phase by changing the cecal microflora.
UNASSIGNED: A total of 600 1-day-old Ross 308 broilers were randomly divided into two groups with six replicates in each group. Chickens in the control group were fed a basal diet, while chickens in the experimental group were fed a diet supplemented with 2 × 108 CFU/kg of C. butyricum and 1 × 109 CFU/kg of B. subtilis. The experimental period was 21 days.
UNASSIGNED: Addition of C. butyricum and B. subtilis significantly increased (p<0.05) the body weight and liver NADP-malic enzyme (NADP-ME) activity of broilers, enhanced (p<0.05) the average daily gain and average daily feed intake of broilers. However, the addition of C. butyricum and B. subtilis did not significantly affect the concentrations of triglyceride and total cholesterol in the serum, the activities of fatty acid synthase and acetyl-CoA carboxylase in the liver, the total antioxidant capacity, glutathione peroxidase activity and malondialdehyde content in the serum and liver. Besides, microbial analysis revealed that supplementation of C. butyricum and B. subtilis increased (p<0.05) the abundance of Firmicutes such as CHKCI001 and Faecalibacterium, decreased (p<0.05) the abundance of Bacteroidota such as Bacteroides and Alistipes. Spearman correlation analysis confirmed that the above cecal microbiota were closely related to the growth performance of broilers (p<0.05). In addition, simultaneous supplementation of C. butyricum and B. subtilis significant affected (p<0.05) 33 different functional pathways such as lipid metabolism and carbohydrate metabolism. This explains the phenomenon of increased growth performance and liver NADP-ME activity in the probiotics group.
UNASSIGNED: The compatibility of C. butyricum and B. subtilis could improve the growth of broilers during the starter phase by changing the cecal microflora.
摘要：
■本研究旨在确定丁酸梭菌与枯草芽孢杆菌的相容性对生长性能的影响，脂质代谢，启动阶段肉鸡的抗氧化状态和盲肠微生物区系。
■将总共600只1日龄的Ross308肉鸡随机分为两组，每组六个重复。对照组饲喂基础日粮,实验组鸡饲喂添加2×108CFU/kg丁酸梭菌和1×109CFU/kg枯草芽孢杆菌的日粮。实验期为21天。
■添加丁酸梭菌和枯草芽孢杆菌显着增加（p<0.05）肉鸡的体重和肝脏NADP-苹果酸酶（NADP-ME）活性，提高了肉鸡的平均日增重和平均日采食量(p<0.05)。然而,添加丁酸梭菌和枯草芽孢杆菌并没有显著影响血清中甘油三酯和总胆固醇的浓度，脂肪酸合成酶和乙酰辅酶A羧化酶在肝脏中的活性，总抗氧化能力，血清和肝脏中谷胱甘肽过氧化物酶活性和丙二醛含量。此外,微生物分析显示，补充丁酸梭菌和枯草芽孢杆菌增加（p<0.05）Firmicutes的丰度，如CHKCI001和Faecalibacterium，降低（p<0.05）拟杆菌和Alistipes等拟杆菌的丰度。Spearman相关分析证实,上述盲肠菌群与肉鸡生长性能密切相关(p<0.05)。此外,同时补充丁酸梭菌和枯草芽孢杆菌显著影响(p<0.05)33种不同的功能途径,如脂质代谢和碳水化合物代谢。这解释了益生菌组中生长性能和肝脏NADP-ME活性增加的现象。
■丁酸梭菌和枯草芽孢杆菌的相容性可以通过改变盲肠微生物区系来改善启动期肉鸡的生长。
■将总共600只1日龄的Ross308肉鸡随机分为两组，每组六个重复。对照组饲喂基础日粮,实验组鸡饲喂添加2×108CFU/kg丁酸梭菌和1×109CFU/kg枯草芽孢杆菌的日粮。实验期为21天。
■添加丁酸梭菌和枯草芽孢杆菌显着增加（p<0.05）肉鸡的体重和肝脏NADP-苹果酸酶（NADP-ME）活性，提高了肉鸡的平均日增重和平均日采食量(p<0.05)。然而,添加丁酸梭菌和枯草芽孢杆菌并没有显著影响血清中甘油三酯和总胆固醇的浓度，脂肪酸合成酶和乙酰辅酶A羧化酶在肝脏中的活性，总抗氧化能力，血清和肝脏中谷胱甘肽过氧化物酶活性和丙二醛含量。此外,微生物分析显示，补充丁酸梭菌和枯草芽孢杆菌增加（p<0.05）Firmicutes的丰度，如CHKCI001和Faecalibacterium，降低（p<0.05）拟杆菌和Alistipes等拟杆菌的丰度。Spearman相关分析证实,上述盲肠菌群与肉鸡生长性能密切相关(p<0.05)。此外,同时补充丁酸梭菌和枯草芽孢杆菌显著影响(p<0.05)33种不同的功能途径,如脂质代谢和碳水化合物代谢。这解释了益生菌组中生长性能和肝脏NADP-ME活性增加的现象。
■丁酸梭菌和枯草芽孢杆菌的相容性可以通过改变盲肠微生物区系来改善启动期肉鸡的生长。
