Abstract：
Along with the rapid development of cellular biological research in recent years, there has been an urgent need for a high-speed, high-precision method of separating target cells from a highly heterogeneous cell population. Among the various cell separation technologies proposed so far, dielectrophoresis (DEP)-based approaches have shown particular promise because they are noninvasive to cells. We have developed a new DEP-based device to separate large numbers of live and dead cells of the human mammary cell line MCF10A. In this study, we validated the separation performance of this device. The results showed the successful separation of a higher percentage of cells than in previous studies, with a separation efficiency higher than 90%. In the past, there have been no confirmed cases in which a separation rate of over 90% and high-speed processing of a large number of cells were simultaneously achieved. It was shown that the proposed device can process large numbers of cells at high speed and with high accuracy.
摘要：
随着近年来细胞生物学研究的迅速发展,迫切需要高速，从高度异质的细胞群体中分离靶细胞的高精度方法。在迄今为止提出的各种细胞分离技术中，基于介电电泳（DEP）的方法显示出特别的前景，因为它们对细胞是非侵入性的。我们已经开发了一种新的基于DEP的设备来分离人类乳腺细胞系MCF10A的大量活细胞和死细胞。在这项研究中,我们验证了该装置的分离性能。结果表明，成功分离的细胞百分比高于以前的研究，分离效率高于90%。在过去,没有确认的病例可以同时实现90%以上的分离率和大量细胞的高速处理。结果表明，所提出的设备可以高速，高精度地处理大量的细胞。
