Abstract：
A variety of LSD analogs have emerged in recent years with dual purposes of avoiding prosecution from possession while providing new options for those willing to experiment with hallucinogenic drugs. In this study, a previously published automated sample preparation method for LSD and its primary metabolite (OH-LSD) was utilized to extract LSD, OH-LSD, and nine LSD analogs from urine. The liquid chromatography tandem mass spectrometry (LC-MS/MS) method was modified from the previously published LC conditions to utilize a different analytical column and gradient elution program. Mobile phases of 10 mM ammonium formate with 0.1% formic acid in deionized water (mobile phase A) and 0.1% formic acid in methanol (mobile phase B) were employed. The method was validated to ANSI/ASB Standard 036 with a 0.1 ng/mL limit of detection for all analytes and was utilized for the analysis of 325 urine specimens. Although no LSD analogs were observed in the samples analyzed, this validated method was demonstrated to be suitable for the analysis of these compounds in laboratories seeking to expand their testing scope. Automated sample preparation allows for the efficient analysis of these analytically challenging compounds with minimal manual handling. Additionally, there was no increased analytical time burden when the LC column and gradient were modified to target nine additional analytes. Detection may improve as new reference standards are developed to allow laboratories to focus on the metabolic products of these analogs. For now, this validated procedure can assist with the routine analysis and surveillance of these emerging substances.
摘要：
近年来出现了各种LSD类似物，其双重目的是避免起诉，同时为那些愿意尝试迷幻药物的人提供新的选择。在这项研究中,先前公布的LSD及其主要代谢物（OH-LSD）的自动样品制备方法用于提取LSD，OH-LSD,和九种尿液中的LSD类似物。液相色谱串联质谱(LC-MS/MS)方法从先前公开的LC条件进行了修改,以利用不同的分析柱和梯度洗脱程序。使用IOmM甲酸铵的流动相,其中0.1%甲酸在去离子水中(流动相A)和0.1%甲酸在甲醇中(流动相B)。该方法根据ANSI/ASB标准036进行了验证，对所有分析物的检测限为0.1ng/mL，并用于分析325个尿液样本。尽管在分析的样品中未观察到LSD类似物，这种经过验证的方法被证明适合在试图扩大其测试范围的实验室中分析这些化合物。自动化样品制备允许以最少的手动处理有效分析这些具有分析挑战性的化合物。此外,当LC柱和梯度被修饰以靶向另外9种分析物时,没有增加分析时间负担。随着开发新的参考标准以允许实验室专注于这些类似物的代谢产物，检测可能会有所改善。现在,这个经过验证的程序可以帮助对这些新出现的物质进行常规分析和监测.
