PDF(Pubmed)
Abstract：
The structures of the Fc base of various IgG antibodies have been examined with a view to understanding how this region can be used to conjugate IgG to nanoparticles. The base structure is found to be largely consistent across a range of species and subtypes, comprising a hydrophobic region surrounded by hydrophilic residues, some of which are charged at physiological conditions. In addition, atomistic Molecular Dynamics simulations were performed to explore how model nanoparticles interact with the base using neutral and negatively charged gold nanoparticles. Both types of nanoparticle interacted readily with the base, leading to an adaptation of the antibody base surface to enhance the interactions. Furthermore, these interactions left the rest of the domain at the base of the Fc region structurally intact. This implies that coupling nanoparticles to the base of an IgG molecule is both feasible and desirable, since it leaves the antibody free to interact with its surroundings so that antigen-binding functionality can be retained. These results will therefore help guide future attempts to develop new nanotechnologies that exploit the unique properties of both antibodies and nanoparticles.
摘要：
已经检查了各种IgG抗体的Fc碱基的结构,以了解该区域如何可用于将IgG缀合至纳米颗粒。发现基本结构在一系列物种和亚型中基本一致，包含由亲水残基包围的疏水区，其中一些是在生理条件下充电的。此外,进行了原子分子动力学模拟，以探索模型纳米粒子如何使用中性和带负电荷的金纳米粒子与碱相互作用。两种类型的纳米粒子都容易与碱相互作用，导致抗体基础表面的适应以增强相互作用。此外，这些相互作用使结构域的其余部分在Fc区的底部在结构上完整。这意味着将纳米颗粒与IgG分子的碱基偶联是可行的和合乎需要的。因为它使抗体自由地与其周围环境相互作用,从而可以保留抗原结合功能。因此，这些结果将有助于指导未来开发新的纳米技术，利用抗体和纳米颗粒的独特特性。
