PDF(Pubmed)
Abstract：
The adoption of the European Green Deal will limit acaricide use in high value crops like raspberry, to be replaced by biological control and other alternative strategies. More basic knowledge on mites in such crops is then necessary, like species, density, and their role as vectors of plant diseases. This study had four aims, focusing on raspberry leaves at northern altitude: (1) identify mite species; (2) study mite population densities; (3) investigate mite intra-plant distribution; (4) investigate co-occurrence of phytophagous mites, raspberry leaf blotch disorder and raspberry leaf blotch virus (RLBV). Four sites in south-eastern Norway were sampled five times. Floricanes from different parts of the sites were collected, taking one leaf from each of the upper, middle, and bottom zones of the cane. Mites were extracted with a washing technique and processed for species identification and RLBV detection. Mites and leaves were tested for RLBV by reverse transcription polymerase chain reaction (RT-PCR) with virus-specific primers. Phytophagous mites, Phyllocoptes gracilis, Tetranychus urticae, and Neotetranychus rubi, and predatory mites, Anystis baccarum and Typhlodromus (Typhlodromus) pyri were identified. All phytophagous mites in cultivated raspberry preferred the upper zone of floricanes, while in non-cultivated raspberry, they preferred the middle zone. The presence of phytophagous mites did not lead to raspberry leaf blotch disorder during this study. RLBV was detected in 1.3% of the sampled plants, none of them with leaf blotch symptoms, and in 4.3% of P. gracilis samples, and in some spider mite samples, implying that Tetranychids could also be vectors of RLBV.
摘要：
欧洲绿色协议的采用将限制杀螨剂在树莓等高价值作物中的使用，被生物控制和其他替代策略所取代。那么需要更多有关此类作物中螨虫的基本知识，像物种一样，密度,以及它们作为植物病害媒介的作用。这项研究有四个目标，关注北方海拔高度的覆盆子叶：（1）确定螨种类；（2）研究螨种群密度；（3）调查螨在植物内的分布；（4）调查植物吸食螨的共同发生，覆盆子叶斑病和覆盆子叶斑病病毒（RLBV）。对挪威东南部的四个地点进行了五次采样。收集了不同地点的植物，从每个上部取一片叶子，中间,和甘蔗的底部区域。用洗涤技术提取螨，并进行处理以进行物种鉴定和RLBV检测。通过使用病毒特异性引物的逆转录聚合酶链反应（RT-PCR）测试螨和叶的RLBV。植物吸食螨，Phyllocoptesgracilis,荨麻疹,和Neotetranychusrubi,和掠食性螨虫,鉴定了任何杆菌属和伤寒（伤寒）。栽培树莓中的所有植食性螨都喜欢氟烷的上部区域，而在非栽培树莓中，他们更喜欢中间区域。在这项研究中，植食性螨的存在并未导致覆盆子叶斑病。在1.3%的采样植物中检测到RLBV，他们都没有叶斑病症状，在4.3%的草原虫样本中，在一些蜘蛛螨样本中，暗示四细胞也可能是RLBV的载体。
