Abstract：
Waterborne pathogens invariably present considerable threats to public health. The quorum sensing (QS) system is instrumental in coordinating bacterial growth and metabolisms. However, the responses and regulatory mechanisms of bacteria to various disinfection technologies through quorum sensing are still unclear. This study examines the inactivation effect of chlorination and ozonation on biofilms and planktonic cells of QS signaling-deficient mutants of Pseudomonas aeruginosa. Cell counting and viability assessment revealed that the combined disinfection of chlorine and ozone was the most effective for inactivating planktonic P. aeruginosa within 10 min of exposure. Additionally, microfluidic chip culture demonstrated that the secretion of quinolone signals escalated biofilms\' disinfection resistance. Disinfection exposure significantly altered the gene expression of wild-type strains and QS signaling-deficient mutants. Moreover, the QS system triggered multilayered gene expression programs as a responsive protection to disinfectant exposure, including oxidative stress, ribosome synthesis, and the nutrient absorption of bacteria. These insights broaden our understanding of bacterial QS in response to disinfection, promising potential strategies toward efficient disinfection processes.
摘要：
水传播病原体总是对公众健康构成相当大的威胁。群体感应（QS）系统有助于协调细菌的生长和代谢。然而,细菌通过群体感应对各种消毒技术的反应和调节机制尚不清楚。这项研究研究了氯化和臭氧化对铜绿假单胞菌QS信号缺乏突变体的生物膜和浮游细胞的灭活作用。细胞计数和活力评估表明，氯和臭氧的联合消毒对于在暴露后10分钟内灭活浮游铜绿假单胞菌最有效。此外,微流控芯片培养表明，喹诺酮类药物信号的分泌增强了生物膜的消毒抗性。消毒暴露显着改变了野生型菌株和QS信号缺乏突变体的基因表达。此外，QS系统触发了多层基因表达程序，作为对消毒剂暴露的响应性保护，包括氧化应激,核糖体合成，和细菌的营养吸收。这些见解扩大了我们对细菌QS对消毒的反应的理解，有希望的潜在策略，以实现有效的消毒过程。
