PDF(Pubmed)
Abstract：
OBJECTIVE: To overcome the natural visual consequences of the physiological aging process, the use of biodegradable fillers made of hyaluronic acid or sodium carboxymethyl cellulose is increasingly popular in modern esthetic medicine. Clinicians can choose from a wide range of fillers with variable compositions and rheological properties, and therefore with different application areas and injection depths. The aim of this study was to analyze and compare the most commonly used fillers for facial augmentation regarding their in vitro biocompatibility and to find potential correlations to their rheological properties.
METHODS: In the present study, direct and indirect in vitro cytotoxicity analysis according to DIN EN ISO 10993-5 were performed on 39 different filler materials for facial augmentation.
RESULTS: All fillers analyzed in this study overall showed satisfactory results in the direct and indirect cytocompatibility tests. While no material was outside the threshold values in the 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide (XTT) cell viability and bromodeoxyuridine (BrdU) cell proliferation assays or in the live-dead staining, only 7 out of the 39 fillers reached the required values in the lactate dehydrogenase assay.
CONCLUSIONS: All biodegradable fillers examined in this study were found to be sufficiently cytocompatible. Although the qualitative analysis of the test results showed differences between the fillers, no concrete correlation between test performance and composition or manufacturer of the fillers was found. Future efforts are required to provide clinicians with even better support in choosing the right filler for optimal outcome and patient satisfaction.
METHODS: In the present study, direct and indirect in vitro cytotoxicity analysis according to DIN EN ISO 10993-5 were performed on 39 different filler materials for facial augmentation.
RESULTS: All fillers analyzed in this study overall showed satisfactory results in the direct and indirect cytocompatibility tests. While no material was outside the threshold values in the 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide (XTT) cell viability and bromodeoxyuridine (BrdU) cell proliferation assays or in the live-dead staining, only 7 out of the 39 fillers reached the required values in the lactate dehydrogenase assay.
CONCLUSIONS: All biodegradable fillers examined in this study were found to be sufficiently cytocompatible. Although the qualitative analysis of the test results showed differences between the fillers, no concrete correlation between test performance and composition or manufacturer of the fillers was found. Future efforts are required to provide clinicians with even better support in choosing the right filler for optimal outcome and patient satisfaction.
摘要：
目的：为了克服生理衰老过程的自然视觉后果，使用由透明质酸或羧甲基纤维素钠制成的可生物降解填充剂在现代美学医学中越来越受欢迎。临床医生可以从多种具有可变组成和流变特性的填充剂中进行选择，因此具有不同的应用领域和注射深度。这项研究的目的是分析和比较最常用的面部增强填充剂的体外生物相容性，并发现其流变特性的潜在相关性。
方法：在本研究中，根据DINENISO10993-5，对39种不同的填充材料进行了直接和间接的体外细胞毒性分析。
结果：本研究中分析的所有填充剂在直接和间接细胞相容性测试中总体显示出令人满意的结果。虽然在2,3-双-（2-甲氧基-4-硝基-5-磺苯基）-（2H）-四唑鎓-5-甲酰苯胺（XTT）细胞活力和溴脱氧尿苷（BrdU）细胞增殖试验中或在活死染色中没有材料超出阈值，在乳酸脱氢酶测定中，39个填料中只有7个达到了所需的值。
结论:发现本研究中检查的所有可生物降解的填充剂是足够细胞相容的。尽管对测试结果的定性分析表明填料之间存在差异，没有发现测试性能与填料组成或制造商之间的具体相关性。未来需要努力为临床医生提供更好的支持，以选择正确的填充物，以获得最佳结果和患者满意度。
方法：在本研究中，根据DINENISO10993-5，对39种不同的填充材料进行了直接和间接的体外细胞毒性分析。
结果：本研究中分析的所有填充剂在直接和间接细胞相容性测试中总体显示出令人满意的结果。虽然在2,3-双-（2-甲氧基-4-硝基-5-磺苯基）-（2H）-四唑鎓-5-甲酰苯胺（XTT）细胞活力和溴脱氧尿苷（BrdU）细胞增殖试验中或在活死染色中没有材料超出阈值，在乳酸脱氢酶测定中，39个填料中只有7个达到了所需的值。
结论:发现本研究中检查的所有可生物降解的填充剂是足够细胞相容的。尽管对测试结果的定性分析表明填料之间存在差异，没有发现测试性能与填料组成或制造商之间的具体相关性。未来需要努力为临床医生提供更好的支持，以选择正确的填充物，以获得最佳结果和患者满意度。
