Abstract：
Integrated wound care through sequentially promoting hemostasis, sealing, and healing holds great promise in clinical practice. However, it remains challenging for regular bioadhesives to achieve integrated care of dynamic wounds due to the difficulties in adapting to dynamic mechanical and wet wound environments. Herein, we reported a type of dehydrated, physical double crosslinked microgels (DPDMs) which were capable of in situ forming highly stretchable, compressible and tissue-adhesive hydrogels for integrated care of dynamic wounds. The DPDMs were designed by the rational integration of the reversible crosslinks and double crosslinks into micronized gels. The reversible physical crosslinks enabled the DPDMs to integrate together, and the double crosslinked characteristics further strengthen the formed macroscopical networks (DPDM-Gels). We demonstrated that the DPDM-Gels simultaneously possess outstanding tensile (∼940 kJ/m3) and compressive (∼270 kJ/m3) toughness, commercial bioadhesives-comparable tissue-adhesive strength, together with stable performance under hundreds of deformations. In vivo results further revealed that the DPDM-Gels could effectively stop bleeding in various bleeding models, even in an actual dynamic environment, and enable the integrated care of dynamic skin wounds. On the basis of the remarkable mechanical and appropriate adhesive properties, together with impressive integrated care capacities, the DPDM-Gels may provide a new approach for the smart care of dynamic wounds. STATEMENT OF SIGNIFICANCE: Integrated care of dynamic wounds holds great significance in clinical practice. However, the dynamic and wet wound environments pose great challenges for existing hydrogels to achieve it. This work developed robust adhesive hydrogels for integrated care of dynamic wounds by designing dehydrated, physical double crosslinked microgels (DPDMs). The reversible and double crosslinks enabled DPDMs to integrate into macroscopic hydrogels with high mechanical properties, appropriate adhesive strength and stable performance under hundreds of external deformations. Upon application at the injury site, DPDM-Gels efficiently stopped bleeding, even in an actual dynamic environment and showed effectiveness in integrated care of dynamic wounds. With the fascinating properties, DPDMs may become an effective tool for smart wound care.
摘要：
通过顺序促进止血的综合伤口护理，密封,治疗在临床实践中有着巨大的希望。然而,由于难以适应动态机械和湿伤口环境,常规生物粘合剂实现动态伤口的综合护理仍然具有挑战性。在这里，我们报道了一种脱水，物理双交联微凝胶（DPDMs）能够原位形成高度可拉伸，用于动态伤口综合护理的可压缩和组织粘附水凝胶。通过将可逆交联和双交联合理地整合到微粉化凝胶中来设计DPDM。可逆的物理交联使DPDM能够集成在一起，和双重交联特性进一步加强形成的宏观网络（DPDM-凝胶）。我们证明了DPDM凝胶同时具有出色的拉伸(~940kJ/m3)和压缩(~270kJ/m3)韧性，商业生物粘合剂-可比的组织粘附强度，在数百种变形下性能稳定。体内实验结果进一步表明，DPDM-Gels在各种出血模型中均能有效止血，即使在实际的动态环境中，并实现动态皮肤伤口的综合护理。基于卓越的机械性能和适当的粘合性能，加上令人印象深刻的综合护理能力，DPDM凝胶可以为动态伤口的智能护理提供新的方法。重要声明：动态伤口的综合护理在临床实践中具有重要意义。然而,动态和潮湿的伤口环境对现有的水凝胶实现它提出了巨大的挑战。这项工作开发了强大的粘合剂水凝胶，用于通过设计脱水来综合护理动态伤口，物理双交联微凝胶(DPDMs)。可逆和双交联使DPDMs能够整合到具有高机械性能的宏观水凝胶中，适当的粘合强度和稳定的性能在数百种外部变形。在受伤部位申请时，DPDM凝胶有效止血，即使在实际的动态环境中，在动态伤口的综合护理中也显示出有效性。有了迷人的特性，DPDMs可能成为智能伤口护理的有效工具。
