Abstract：
DNA markers are used as a size reference and sample loading control during gel electrophoresis. Most markers are designed for conventional gel electrophoresis to separate DNA smaller than 20 kb. For larger molecules, pulsed-field gel electrophoresis (PFGE) marker is required. Limited PFGE markers are available because large DNA are prone to nicking and degradation, causing smeary bands. Here, we developed a robust marker based on bacterial artificial chromosomes (BACs) with bands up to 184 kb. This marker could consistently confer intense and distinct bands for accurate gel analysis in molecular biology studies, laboratory validations or clinical diagnosis.
摘要：
在凝胶电泳期间,DNA标记用作大小参考和样品加载对照。大多数标记物被设计用于常规凝胶电泳以分离小于20kb的DNA。对于较大的分子，脉冲场凝胶电泳（PFGE）标记是必需的。有限的PFGE标记是可用的，因为大的DNA容易产生切口和降解，造成污点带。这里,我们开发了一种基于细菌人工染色体（BAC）的强大标记，其条带高达184kb。该标记可以始终赋予强烈而独特的条带，以便在分子生物学研究中进行准确的凝胶分析，实验室验证或临床诊断。
