Abstract：
Fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a global pest causing damage to several crops. However, its management using chemical control is a challenge due to its capacity to evolve resistance to insecticides. After 6 generations of selection with lambda-cyhalothrin, the LC50 for the insecticide-resistant strain (Lambda-Sel) was 486 ppm, higher than that of the field strain (FAW-MUL) (7.5 ppm), susceptible laboratory strain (Lab-PK) (0.46 ppm), and laboratory unselected strain (UNSEL) (5.26 ppm). Laboratory selection with lambda-cyhalothrin increased resistance from 16.3- to 1056.52-fold and 1.43- to 92.4-fold to lambda-cyhalothrin compared to Lab-PK and UNSEL strains, respectively. The selected strain of S. frugiperda (Lambda-Sel) presented low cross-resistance to chlorpyrifos, moderate to deltamethrin and indoxacarb, very low to spinosad, and no cross-resistance to emamectin benzoate. The realized heritability (h2) of lambda-cyhalothrin resistance in the Lambda-Sel strain was very high (0.88). The reciprocal cross progenies of F1 (Lambda-Sel ♀ × Lab-PK ♂), F1\' (Lambda-Sel ♂ × Lab-PK ♀), BC1 (F1 ♀ × Lambda-Sel ♂), and BC2 (F1 ♀ × Lab-PK ♂) showed high resistance ratios of 545.64-, 396.52-, 181.18-, and 146.54-fold, respectively compared to Lab-PK. The degree of dominance values for lambda-cyhalothrin in F1 and F1\' indicates incompletely dominant resistance. The difference between observed and expected mortality in backcross populations (BC1 and BC2) revealed a polygenic resistance. In conclusion, the resistance to lambda-cyhalothrin was autosomal, incompletely dominant, and polygenic. These findings provide new insights for insect resistance management strategies to mitigate the occurrence of resistance in this global pest.
摘要：
秋季粘虫（FAW）斜纹夜蛾（J.E.Smith）（鳞翅目：夜蛾科），是一种对几种作物造成损害的全球性害虫。然而,由于其对杀虫剂产生抗药性的能力，因此使用化学控制进行管理是一项挑战。经过6代高效氯氰菊酯的选择，抗杀虫剂菌株（Lambda-Sel）的LC50为486ppm，高于现场应变(FAW-MUL)(7.5ppm)，易感实验室菌株（Lab-PK）（0.46ppm），和实验室未选择的菌株(UNSEL)(5.26ppm)。与Lab-PK和UNSEL菌株相比，使用氯氟氰菊酯的实验室选择对氯氟氰菊酯的抗性从16.3倍增加到1056.52倍，从1.43倍增加到92.4倍，分别。选定的S.frugiperda（Lambda-Sel）菌株对毒死蜱的交叉抗性较低，中度溴氰菊酯和吲哚沙卡威，非常低的多杀菌素，对甲氨基阿维菌素苯甲酸酯没有交叉抗性。Lambda-Sel菌株中λ-氯氟氰菊酯抗性的实现遗传力（h2）非常高（0.88）。F1的相互杂交后代(Lambda-Sel‰×Lab-PK‰)，F1\'(Lambda-Sel‰×Lab-PK‰),BC1(F1‰×Lambda-Sel‰),和BC2（F1‰×Lab-PK‰）显示出545.64-的高电阻比，396.52-,181.18-,146.54倍，分别与Lab-PK进行比较。F1和F1'中lambda-氯氟氰菊酯的优势度表明不完全显性抗性。回交种群（BC1和BC2）中观察到的死亡率与预期死亡率之间的差异显示出多基因抗性。总之,对氯氟氰菊酯的抗性是常染色体，不完全占优势，和多基因。这些发现为昆虫抗性管理策略提供了新的见解，以减轻这种全球害虫中抗性的发生。
