Abstract：
Cutibacterium acnes is the most abundant bacterium of the human skin microbiome since adolescence, participating in both skin homeostasis and diseases. Here, we demonstrate individual and niche heterogeneity of C. acnes from 1,234 isolate genomes. Skin disease (atopic dermatitis and acne) and body site shape genomic differences of C. acnes, stemming from horizontal gene transfer and selection pressure. C. acnes harbors characteristic metabolic functions, fewer antibiotic resistance genes and virulence factors, and a more stable genome compared with Staphylococcus epidermidis. Integrated genome, transcriptome, and metabolome analysis at the strain level unveils the functional characteristics of C. acnes. Consistent with the transcriptome signature, C. acnes in a sebum-rich environment induces toxic and pro-inflammatory effects on keratinocytes. L-carnosine, an anti-oxidative stress metabolite, is up-regulated in the C. acnes metabolome from atopic dermatitis and attenuates skin inflammation. Collectively, our study reveals the joint impact of genes and the microenvironment on C. acnes function.
摘要：
粉刺杆菌是青春期以来人类皮肤微生物组最丰富的细菌，参与皮肤稳态和疾病。这里,我们从1,234个分离基因组中证明了痤疮丙酸杆菌的个体和生态位异质性。皮肤病（特应性皮炎和痤疮）和身体部位形状基因组的分歧。痤疮，源于水平基因转移和选择压力。C.痤疮具有特征性的代谢功能，较少的抗生素抗性基因和毒力因子，与表皮葡萄球菌相比，基因组更稳定。整合的基因组，转录组，菌株水平的代谢组分析揭示了痤疮梭菌的功能特征。与转录组签名一致，富含皮脂的环境中的痤疮丙酸杆菌对角质形成细胞诱导毒性和促炎作用。L-肌肽,抗氧化应激代谢产物,在特应性皮炎的痤疮丙酸杆菌代谢组中上调并减轻皮肤炎症。总的来说，我们的研究揭示了基因和微环境对痤疮丙酸杆菌功能的共同影响。
